poly(UG)-tailed RNAs in genome protection and epigenetic inheritance

doi:10.1038/s41586-020-2323-8

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2323-8
	poly(UG)-tailed RNAs in genome protection and epigenetic inheritance
	Raj, Dipak K.1,2; Das Mohapatra, Alok 1,2; Jnawali, Anup 1,2; Zuromski, Jenna 1,2; Jha, Ambrish 1; Cham-Kpu, Gerald 1,2; Sherman, Brett 1; Rudlaff, Rachel M.3,4; Nixon, Christina E.1,2; Hilton, Nicholas 1,19; Oleinikov, Andrew V.5; Chesnokov, Olga 5; Merritt, Jordan 5; Pond-Tor, Sunthorn 1,2
	2020-04-22
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	582 期号:7811 页码:283-+
文章类型	Article
语种	英语
国家	USA; Peoples R China
英文关键词	Mobile genetic elements threaten genome integrity in all organisms. RDE-3 (also known as MUT-2) is a ribonucleotidyltransferase that is required for transposon silencing and RNA interference in Caenorhabditis elegans(1-4). When tethered to RNAs in heterologous expression systems, RDE-3 can add long stretches of alternating non-templated uridine (U) and guanosine (G) ribonucleotides to the 3 ' termini of these RNAs (designated poly(UG) or pUG tails)(5). Here we show that, in its natural context in C. elegans, RDE-3 adds pUG tails to targets of RNA interference, as well as to transposon RNAs. RNA fragments attached to pUG tails with more than 16 perfectly alternating 3 ' U and G nucleotides become gene-silencing agents. pUG tails promote gene silencing by recruiting RNA-dependent RNA polymerases, which use pUG-tailed RNAs (pUG RNAs) as templates to synthesize small interfering RNAs (siRNAs). Our results show that cycles of pUG RNA-templated siRNA synthesis and siRNA-directed pUG RNA biogenesis underlie double-stranded-RNA-directed transgenerational epigenetic inheritance in the C. elegans germline. We speculate that this pUG RNA-siRNA silencing loop enables parents to inoculate progeny against the expression of unwanted or parasitic genetic elements. In Caenorhabditis elegans, the ribonucleotidyltransferase RDE-3 adds alternating uridine and guanosine ribonucleotides to the 3 ' termini of RNAs, a key step in RNA interference and thus epigenetic inheritance in the C. elegans germline.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000534444800002
WOS关键词	DOUBLE-STRANDED-RNA ; NUCLEOTIDYLTRANSFERASE SUPERFAMILY ; OOCYTE MATURATION ; GERM-LINE ; C-ELEGANS ; GENE ; INTERFERENCE ; BINDING ; RDE-1 ; AMPLIFICATION
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281545
专题	地球科学资源环境科学气候变化
作者单位	1.Brown Univ, Rhode Isl Hosp, Med Sch, Ctr Int Hlth Res, Providence, RI 02903 USA; 2.Brown Univ, Dept Pathol & Lab Med, Med Sch, Providence, RI 02912 USA; 3.Boston Childrens Hosp, Div Infect Dis, Boston, MA USA; 4.Harvard Med Sch, Dept Pediat, Boston, MA 02115 USA; 5.Florida Atlantic Univ, Charles E Schmidt Coll Med, Boca Raton, FL 33431 USA; 6.Yale Univ, Dept Internal Med, New Haven, CT USA; 7.Yale Univ, Dept Microbial Pathogenesis, New Haven, CT USA; 8.Seattle Biomed Res Inst, Mother Offspring Malaria Studies MOMS Project, 4 Nickerson St, Seattle, WA 98109 USA; 9.Muheza Designated Dist Hosp, Muheza, Tanzania; 10.Muhimbili Univ Hlth & Allied Sci, Dar Es Salaam, Tanzania; 11.Ctr Dis Control & Prevent, Infect Dis Pathol Branch, Atlanta, GA USA; 12.NIAID, Lab Malaria Immunol & Vaccinol, NIH, Rockville, MD USA; 13.Columbia Univ, Irving Med Ctr, Dept Microbiol & Immunol, New York, NY USA; 14.Columbia Univ, Div Infect Dis, Irving Med Ctr, Dept Med, New York, NY USA; 15.Univ Seoul, Grad Sch Urban Publ Hlth, Seoul, South Korea; 16.Univ Penn, Dept Med, Philadelphia, PA 19104 USA; 17.Acuitas Therapeut, Vancouver, BC, Canada; 18.Brown Univ, Rhode Isl Hosp, Dept Pediat, Med Sch, Providence, RI 02903 USA; 19.Univ Calif Davis, Dept Microbiol & Mol Genet, Davis, CA 95616 USA
推荐引用方式 GB/T 7714	Raj, Dipak K.,Das Mohapatra, Alok,Jnawali, Anup,et al. poly(UG)-tailed RNAs in genome protection and epigenetic inheritance[J]. NATURE,2020,582(7811):283-+.
APA	Raj, Dipak K..,Das Mohapatra, Alok.,Jnawali, Anup.,Zuromski, Jenna.,Jha, Ambrish.,...&Pond-Tor, Sunthorn.(2020).poly(UG)-tailed RNAs in genome protection and epigenetic inheritance.NATURE,582(7811),283-+.
MLA	Raj, Dipak K.,et al."poly(UG)-tailed RNAs in genome protection and epigenetic inheritance".NATURE 582.7811(2020):283-+.