The stepwise assembly of the neonatal virome is modulated by breastfeeding

doi:10.1038/s41586-020-2192-1

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2192-1
	The stepwise assembly of the neonatal virome is modulated by breastfeeding
	Medina, Christopher B.1,2; Mehrotra, Parul 3; Arandjelovic, Sanja 1,2; Perrys, Justin S. A.1,2; Guo, Yizhan 4; Morioka, Sho 1,2; Barron, Brady 1,5; Walk, Scott F.1,2; Ghesquiere, Bart 6; Lorenz, Ulrike 4,7; Krupnick, Alexander S.5,7; Ravichandran, Kodi S.1,2,3
	2020-03-18
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
文章类型	Article;Early Access
语种	英语
国家	USA; Botswana
英文关键词	The infant gut is colonized first by temperate bacteriophages induced from pioneer bacteria and later by viruses that replicate in human cells, the populations of which are modulated by breastfeeding. The gut of healthy human neonates is usually devoid of viruses at birth, but quickly becomes colonized, which-in some cases-leads to gastrointestinal disorders(1-4). Here we show that the assembly of the viral community in neonates takes place in distinct steps. Fluorescent staining of virus-like particles purified from infant meconium or early stool samples shows few or no particles, but by one month of life particle numbers increase to 10(9) per gram, and these numbers seem to persist throughout life(5-7). We investigated the origin of these viral populations using shotgun metagenomic sequencing of virus-enriched preparations and whole microbial communities, followed by targeted microbiological analyses. Results indicate that, early after birth, pioneer bacteria colonize the infant gut and by one month prophages induced from these bacteria provide the predominant population of virus-like particles. By four months of life, identifiable viruses that replicate in human cells become more prominent. Multiple human viruses were more abundant in stool samples from babies who were exclusively fed on formula milk compared with those fed partially or fully on breast milk, paralleling reports that breast milk can be protective against viral infections(8-10). Bacteriophage populations also differed depending on whether or not the infant was breastfed. We show that the colonization of the infant gut is stepwise, first mainly by temperate bacteriophages induced from pioneer bacteria, and later by viruses that replicate in human cells this second phase is modulated by breastfeeding.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000534394600001
WOS关键词	HUMAN-MILK ; DYNAMICS ; VIRUSES ; DIVERSITY ; SAPOVIRUS ; DIARRHEA ; INFANTS ; DEATHS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281528
专题	地球科学资源环境科学气候变化
作者单位	1.Univ Virginia, Ctr Cell Clearance, Charlottesville, VA 22904 USA; 2.Univ Virginia, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22904 USA; 3.Univ Ghent, VIB UGent Inflammat Res Ctr, Biomed Mol Biol, Ghent, Belgium; 4.Univ Virginia, Dept Surg, Charlottesville, VA USA; 5.Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USA; 6.Katholieke Univ Leuven, Dept Oncol & VIB, Leuven, Belgium; 7.Univ Virginia, Carter Immunol Ctr, Charlottesville, VA USA
推荐引用方式 GB/T 7714	Medina, Christopher B.,Mehrotra, Parul,Arandjelovic, Sanja,et al. The stepwise assembly of the neonatal virome is modulated by breastfeeding[J]. NATURE,2020.
APA	Medina, Christopher B..,Mehrotra, Parul.,Arandjelovic, Sanja.,Perrys, Justin S. A..,Guo, Yizhan.,...&Ravichandran, Kodi S..(2020).The stepwise assembly of the neonatal virome is modulated by breastfeeding.NATURE.
MLA	Medina, Christopher B.,et al."The stepwise assembly of the neonatal virome is modulated by breastfeeding".NATURE (2020).