Constructing protein polyhedra via orthogonal chemical interactions

doi:10.1038/s41586-019-1928-2

GSTDTAP > 地球科学

DOI	10.1038/s41586-019-1928-2
	Constructing protein polyhedra via orthogonal chemical interactions
	Mooley, K. P.; Deller, A. T.; Gottlieb, O.; Nakar, E.; Hallinan, G.; Bourke, S.; Frail, D. A.; Horesh, A.; Corsi, A.; Hotokezaka, K.
	2020-01-09
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	578 期号:7793 页码:172-+
文章类型	Article
语种	英语
国家	USA
英文关键词	Many proteins exist naturally as symmetrical homooligomers or homopolymers(1). The emergent structural and functional properties of such protein assemblies have inspired extensive efforts in biomolecular design(2-5). As synthesized by ribosomes, proteins are inherently asymmetric. Thus, they must acquire multiple surface patches that selectively associate to generate the different symmetry elements needed to form higher-order architectures(1,6)-a daunting task for protein design. Here we address this problem using an inorganic chemical approach, whereby multiple modes of protein-protein interactions and symmetry are simultaneously achieved by selective, ' one-pot' coordination of soft and hard metal ions. We show that a monomeric protein (protomer) appropriately modified with biologically inspired hydroxamate groups and zinc-binding motifs assembles through concurrent Fe3+ and Zn2+ coordination into discrete dodecameric and hexameric cages. Our cages closely resemble natural polyhedral protein architectures(7,8) and are, to our knowledge, unique among designed systems(9-13) in that they possess tightly packed shells devoid of large apertures. At the same time, they can assemble and disassemble in response to diverse stimuli, owing to their heterobimetallic construction on minimal interprotein-bonding footprints. With stoichiometries ranging from [2 Fe:9 Zn:6 protomers] to [8 Fe:21 Zn:12 protomers], these protein cages represent some of the compositionally most complex protein assemblies-or inorganic coordination complexes-obtained by design. An inorganic chemical approach to biomolecular design is used to generate ' cages' that can simultaneously promote symmetry and multiple modes of protein interactions.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000508801100003
WOS关键词	COORDINATION CHEMISTRY ; DESIGN ; CRYSTAL ; CAGES ; VISUALIZATION ; STABILITY ; DYNAMICS ; SYMMETRY ; MODEL
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281441
专题	地球科学资源环境科学气候变化
推荐引用方式 GB/T 7714	Mooley, K. P.,Deller, A. T.,Gottlieb, O.,et al. Constructing protein polyhedra via orthogonal chemical interactions[J]. NATURE,2020,578(7793):172-+.
APA	Mooley, K. P..,Deller, A. T..,Gottlieb, O..,Nakar, E..,Hallinan, G..,...&Hotokezaka, K..(2020).Constructing protein polyhedra via orthogonal chemical interactions.NATURE,578(7793),172-+.
MLA	Mooley, K. P.,et al."Constructing protein polyhedra via orthogonal chemical interactions".NATURE 578.7793(2020):172-+.