DOI | 10.1038/s41586-019-1910-z
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| The structural basis for cohesin-CTCF-anchored loops |
| Li, Yan1; Haarhuis, Judith H. I.2; Sedeno Cacciatore, Angela2; Oldenkamp, Roel2; van Ruiten, Marjon S.2; Willems, Laureen2; Teunissen, Hans3; Muir, Kyle W.1,4; de Wit, Elzo3; Rowland, Benjamin D.2; Panne, Daniel1,5
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| 2020-01-30
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发表期刊 | NATURE
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ISSN | 0028-0836
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EISSN | 1476-4687
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出版年 | 2020
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卷号 | 578期号:7795页码:472-+ |
文章类型 | Article
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语种 | 英语
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国家 | France; Netherlands; England |
英文关键词 | Cohesin catalyses the folding of the genome into loops that are anchored by CTCF1. The molecular mechanism of how cohesin and CTCF structure the 3D genome has remained unclear. Here we show that a segment within the CTCF N terminus interacts with the SA2-SCC1 subunits of human cohesin. We report a crystal structure of SA2-SCC1 in complex with CTCF at a resolution of 2.7 angstrom, which reveals the molecular basis of the interaction. We demonstrate that this interaction is specifically required for CTCF-anchored loops and contributes to the positioning of cohesin at CTCF binding sites. A similar motif is present in a number of established and newly identified cohesin ligands, including the cohesin release factor WAPL(2,3). Our data suggest that CTCF enables the formation of chromatin loops by protecting cohesin against loop release. These results provide fundamental insights into the molecular mechanism that enables the dynamic regulation of chromatin folding by cohesin and CTCF.
The crystal structure of the SA2-SCC1 subunits of human cohesin in complex with CTCF reveals the molecular basis of the cohesin-CTCF interaction that enables the dynamic regulation of chromatin folding.
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领域 | 地球科学
; 气候变化
; 资源环境
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收录类别 | SCI-E
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WOS记录号 | WOS:000511285500001
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WOS关键词 | FULLY-AUTOMATIC CHARACTERIZATION
; HI-C REVEALS
; DATA-COLLECTION
; 3D GENOME
; WAPL
; CHROMOSOMES
; RESOLUTION
; MECHANISM
; TOPOLOGY
; DOMAINS
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WOS类目 | Multidisciplinary Sciences
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WOS研究方向 | Science & Technology - Other Topics
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引用统计 |
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文献类型 | 期刊论文
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条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/281425
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专题 | 地球科学 资源环境科学 气候变化
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作者单位 | 1.European Mol Biol Lab, Grenoble, France; 2.Netherlands Canc Inst, Div Gene Regulat, Amsterdam, Netherlands; 3.Netherlands Canc Inst, Oncode Inst, Div Gene Regulat, Amsterdam, Netherlands; 4.MRC Lab Mol Biol, Cambridge, England; 5.Univ Leicester, Leicester Inst Struct & Chem Biol, Dept Mol & Cell Biol, Leicester, Leics, England
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推荐引用方式 GB/T 7714 |
Li, Yan,Haarhuis, Judith H. I.,Sedeno Cacciatore, Angela,et al. The structural basis for cohesin-CTCF-anchored loops[J].
NATURE,2020,578(7795):472-+.
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APA |
Li, Yan.,Haarhuis, Judith H. I..,Sedeno Cacciatore, Angela.,Oldenkamp, Roel.,van Ruiten, Marjon S..,...&Panne, Daniel.(2020).The structural basis for cohesin-CTCF-anchored loops.NATURE,578(7795),472-+.
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MLA |
Li, Yan,et al."The structural basis for cohesin-CTCF-anchored loops".NATURE 578.7795(2020):472-+.
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