GSTDTAP  > 地球科学
DOI10.1038/s41586-020-1977-6
Phase separation organizes the site of autophagosome formation
Imachi, Hiroyuki1,10; Nobu, Masaru K.2,10; Nakahara, Nozomi1,2,3; Morono, Yuki4; Ogawara, Miyuki1; Takaki, Yoshihiro1; Takano, Yoshinori5; Uematsu, Katsuyuki6; Ikuta, Tetsuro7; Ito, Motoo4; Matsui, Yohei; Miyazaki, Masayuki1; Murata, Kazuyoshi8; Saito, Yumi1; Sakai, Sanae1; Song, Chihong8; Tasumi, Eiji1; Yamanaka, Yuko1; Yamaguchi, Takashi3; Kamagata, Yoichi2; Tamaki, Hideyuki; Takai, Ken1,9
2020
发表期刊NATURE
ISSN0028-0836
EISSN1476-4687
出版年2020
卷号578期号:7794页码:301-+
文章类型Article
语种英语
国家Japan; Germany
英文关键词

The pre-autophagosomal structure in yeast is a liquid-like condensate of Atg proteins whose phase separation may have a critical, active role in autophagy.


Many biomolecules undergo liquid-liquid phase separation to form liquid-like condensates that mediate diverse cellular functions(1,2). Autophagy is able to degrade such condensates using autophagosomes-double-membrane structures that are synthesized de novo at the pre-autophagosomal structure (PAS) in yeast(3-5). Whereas Atg proteins that associate with the PAS have been characterized, the physicochemical and functional properties of the PAS remain unclear owing to its small size and fragility. Here we show that the PAS is in fact a liquid-like condensate of Atg proteins. The autophagy-initiating Atg1 complex undergoes phase separation to form liquid droplets in vitro, and point mutations or phosphorylation that inhibit phase separation impair PAS formation in vivo. In vitro experiments show that Atg1-complex droplets can be tethered to membranes via specific protein-protein interactions, explaining the vacuolar membrane localization of the PAS in vivo. We propose that phase separation has a critical, active role in autophagy, whereby it organizes the autophagy machinery at the PAS.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000511285700009
WOS关键词NONPARAMETRIC-ESTIMATION ; MEMBRANE DYNAMICS ; ATG1 KINASE ; PROTEIN ; COMPLEX ; INDUCTION ; CYTOPLASM ; ACTIVATE ; SCAFFOLD ; PORE
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/281326
专题地球科学
资源环境科学
气候变化
作者单位1.Japan Agcy Marine Earth Sci & Technol JAMSTEC, Inst Extra Cutting Edge Sci & Technol Avant Garde, Yokosuka, Kanagawa, Japan;
2.Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki, Japan;
3.Nagaoka Univ Technol, Dept Civil & Environmental Engn, Nagaoka, Niigata, Japan;
4.JAMSTEC, Xstar, Kochi Inst Core Sample Res, Nankoku, Kochi, Japan;
5.JAMSTEC, Res Inst Marine Resources Utilizat, Biogeochem Program, Yokosuka, Kanagawa, Japan;
6.Marine Work Japan, Dept Marine & Earth Sci, Yokosuka, Kanagawa, Japan;
7.JAMSTEC, Res Inst Global Change, Yokosuka, Kanagawa, Japan;
8.JAMSTEC, Res Inst Marine Resources Utilizat, Yokosuka, Kanagawa, Japan;
9.Natl Inst Physiol Sci, Okazaki, Aichi, Japan;
10.Natl Inst Nat Sci, Sect Explorat Life Extreme Environm, Exploratory Res Ctr Life & Living Syst ExCELLS, Okazaki, Aichi, Japan
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GB/T 7714
Imachi, Hiroyuki,Nobu, Masaru K.,Nakahara, Nozomi,et al. Phase separation organizes the site of autophagosome formation[J]. NATURE,2020,578(7794):301-+.
APA Imachi, Hiroyuki.,Nobu, Masaru K..,Nakahara, Nozomi.,Morono, Yuki.,Ogawara, Miyuki.,...&Takai, Ken.(2020).Phase separation organizes the site of autophagosome formation.NATURE,578(7794),301-+.
MLA Imachi, Hiroyuki,et al."Phase separation organizes the site of autophagosome formation".NATURE 578.7794(2020):301-+.
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