Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function

doi:10.1038/s41586-020-2195-y

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2195-y
	Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function
	Kanarek, Naama 1,2,3; Petrova, Boryana 1; Sabatini, David M.3,4,5,6
	2020-03-01
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	580 期号:7805 页码:669-+
文章类型	Article
语种	英语
国家	Germany; England
英文关键词	Cryo-electron microscopy structures of the DNA-binding domains of the pioneer transcription factor SOX2 and its close homologue SOX11 elucidate the role of these factors in initiating chromatin opening and nucleosome remodelling. ' Pioneer' transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming(1,2). Pioneer factors can bind nucleosomal DNA to enable gene expression from regions of the genome with closed chromatin. SOX2 is a prominent pioneer factor that is essential for pluripotency and self-renewal of embryonic stem cells(3). Here we report cryo-electron microscopy structures of the DNA-binding domains of SOX2 and its close homologue SOX11 bound to nucleosomes. The structures show that SOX factors can bind and locally distort DNA at superhelical location 2. The factors also facilitate detachment of terminal nucleosomal DNA from the histone octamer, which increases DNA accessibility. SOX-factor binding to the nucleosome can also lead to a repositioning of the N-terminal tail of histone H4 that includes residue lysine 16. We speculate that this repositioning is incompatible with higher-order nucleosome stacking, which involves contacts of the H4 tail with a neighbouring nucleosome. Our results indicate that pioneer transcription factors can use binding energy to initiate chromatin opening, and thereby facilitate nucleosome remodelling and subsequent transcription.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000528166900003
WOS关键词	CRYO-EM ; TRANSCRIPTION FACTORS ; CRYSTAL-STRUCTURE ; RECOMBINANT HISTONES ; CHROMATIN-STRUCTURE ; COMPLEX SUGGESTS ; MOLECULAR-BASIS ; TAIL DOMAINS ; DNA ; PLURIPOTENCY
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281318
专题	地球科学资源环境科学气候变化
作者单位	1.Boston Childrens Hosp, Dept Pathol, Boston, MA USA; 2.Harvard Med Sch, Boston, MA 02115 USA; 3.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA; 4.Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA; 5.MIT, Howard Hughes Med Inst, Dept Biol, Cambridge, MA USA; 6.Koch Inst Integrat Canc Res, Cambridge, MA USA
推荐引用方式 GB/T 7714	Kanarek, Naama,Petrova, Boryana,Sabatini, David M.. Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function[J]. NATURE,2020,580(7805):669-+.
APA	Kanarek, Naama,Petrova, Boryana,&Sabatini, David M..(2020).Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function.NATURE,580(7805),669-+.
MLA	Kanarek, Naama,et al."Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function".NATURE 580.7805(2020):669-+.