GSTDTAP  > 地球科学
DOI10.1038/s41586-020-2195-y
Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function
Kanarek, Naama1,2,3; Petrova, Boryana1; Sabatini, David M.3,4,5,6
2020-03-01
发表期刊NATURE
ISSN0028-0836
EISSN1476-4687
出版年2020
卷号580期号:7805页码:669-+
文章类型Article
语种英语
国家Germany; England
英文关键词

Cryo-electron microscopy structures of the DNA-binding domains of the pioneer transcription factor SOX2 and its close homologue SOX11 elucidate the role of these factors in initiating chromatin opening and nucleosome remodelling.


' Pioneer' transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming(1,2). Pioneer factors can bind nucleosomal DNA to enable gene expression from regions of the genome with closed chromatin. SOX2 is a prominent pioneer factor that is essential for pluripotency and self-renewal of embryonic stem cells(3). Here we report cryo-electron microscopy structures of the DNA-binding domains of SOX2 and its close homologue SOX11 bound to nucleosomes. The structures show that SOX factors can bind and locally distort DNA at superhelical location 2. The factors also facilitate detachment of terminal nucleosomal DNA from the histone octamer, which increases DNA accessibility. SOX-factor binding to the nucleosome can also lead to a repositioning of the N-terminal tail of histone H4 that includes residue lysine 16. We speculate that this repositioning is incompatible with higher-order nucleosome stacking, which involves contacts of the H4 tail with a neighbouring nucleosome. Our results indicate that pioneer transcription factors can use binding energy to initiate chromatin opening, and thereby facilitate nucleosome remodelling and subsequent transcription.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000528166900003
WOS关键词CRYO-EM ; TRANSCRIPTION FACTORS ; CRYSTAL-STRUCTURE ; RECOMBINANT HISTONES ; CHROMATIN-STRUCTURE ; COMPLEX SUGGESTS ; MOLECULAR-BASIS ; TAIL DOMAINS ; DNA ; PLURIPOTENCY
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/281318
专题地球科学
资源环境科学
气候变化
作者单位1.Boston Childrens Hosp, Dept Pathol, Boston, MA USA;
2.Harvard Med Sch, Boston, MA 02115 USA;
3.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA;
4.Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA;
5.MIT, Howard Hughes Med Inst, Dept Biol, Cambridge, MA USA;
6.Koch Inst Integrat Canc Res, Cambridge, MA USA
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GB/T 7714
Kanarek, Naama,Petrova, Boryana,Sabatini, David M.. Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function[J]. NATURE,2020,580(7805):669-+.
APA Kanarek, Naama,Petrova, Boryana,&Sabatini, David M..(2020).Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function.NATURE,580(7805),669-+.
MLA Kanarek, Naama,et al."Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function".NATURE 580.7805(2020):669-+.
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