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DOI10.1029/2019WR025916
Microbial Community Composition in Deep-Subsurface Reservoir Fluids Reveals Natural Interwell Connectivity
Zhang, Yuran1; Dekas, Anne E.2; Hawkins, Adam J.1,3; Parada, Alma E.2; Gorbatenko, Oxana4; Li, Kewen1; Horne, Roland N.1
2020-02-01
发表期刊WATER RESOURCES RESEARCH
ISSN0043-1397
EISSN1944-7973
出版年2020
卷号56期号:2
文章类型Article
语种英语
国家USA
英文摘要

The identification of natural fractures and the wells they connect is crucial for the development of geological reservoirs because it may have an important impact on reservoir model construction and hydraulic fracture propagation. In this study we investigated the use of a novel data source, the microbial community composition in the reservoir formation fluids, for identification of interwell connectivity caused by natural fractures. We verified this concept at a newly developed mesoscale enhanced geothermal system (EGS) field testbed located 4,850 ft (1,478 m) beneath ground surface at the Sanford Underground Research Facility in Lead, SD. Fluids produced at or near the EGS testbed were sampled and subjected to high-throughput 16S rRNA gene amplicon sequencing to analyze the microbial community profile therein. Despite the typically substantial heterogeneity across the community profiles of samples spatially distributed (10 m to 1.9 km apart) throughout the site, samples from two wells at the EGS testbed showed highly similar microbial community composition, suggesting the two wells intersected the same natural fracture. This evidence of natural connectivity between the two wells at the EGS testbed was later corroborated by core log analysis and sewer camera surveys into the boreholes. Besides the field case described in this study, microbial community composition as a reservoir diagnostic tool would be applicable in a much broader context such as unconventional hydrocarbon exploitation, groundwater reservoir characterization, and environmental remediation, adding valuable "hard" data capable of pinpointing the origins of fluids unambiguously.


英文关键词interwell connectivity microbial community high-throughput DNA sequencing deep subsurface fracture characterization DNA signature
领域资源环境
收录类别SCI-E
WOS记录号WOS:000535672800001
WOS关键词BACTERIAL COMMUNITIES ; BIODIVERSITY ; DIVERSITY ; PATTERNS ; SEQUENCE ; INSIGHTS ; TRACER ; WATER ; LIFE
WOS类目Environmental Sciences ; Limnology ; Water Resources
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/280539
专题资源环境科学
作者单位1.Stanford Univ, Dept Energy Resources Engn, Stanford, CA 94305 USA;
2.Stanford Univ, Dept Earth Syst Sci, Stanford, CA 94305 USA;
3.Stanford Univ, TomKat Ctr Sustainable Energy, Stanford, CA 94305 USA;
4.Black Hills State Univ, Sch Nat Sci, Spearfish, SD 57799 USA
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GB/T 7714
Zhang, Yuran,Dekas, Anne E.,Hawkins, Adam J.,et al. Microbial Community Composition in Deep-Subsurface Reservoir Fluids Reveals Natural Interwell Connectivity[J]. WATER RESOURCES RESEARCH,2020,56(2).
APA Zhang, Yuran.,Dekas, Anne E..,Hawkins, Adam J..,Parada, Alma E..,Gorbatenko, Oxana.,...&Horne, Roland N..(2020).Microbial Community Composition in Deep-Subsurface Reservoir Fluids Reveals Natural Interwell Connectivity.WATER RESOURCES RESEARCH,56(2).
MLA Zhang, Yuran,et al."Microbial Community Composition in Deep-Subsurface Reservoir Fluids Reveals Natural Interwell Connectivity".WATER RESOURCES RESEARCH 56.2(2020).
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