Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.5194/acp-20-1817-2020 |
Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia | |
Negron, Arnaldo1,2; DeLeon-Rodriguez, Natasha3,9; Waters, Samantha M.1,10; Ziemba, Luke D.4; Anderson, Bruce4; Bergin, Michael5; Konstantinidis, Konstantinos T.3,6; Nenes, Athanasios1,7,8 | |
2020-02-14 | |
发表期刊 | ATMOSPHERIC CHEMISTRY AND PHYSICS |
ISSN | 1680-7316 |
EISSN | 1680-7324 |
出版年 | 2020 |
卷号 | 20期号:3页码:1817-1838 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Greece; Switzerland |
英文摘要 | The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation, and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA, over a 2-month period and showed clearly defined populations of nucleic-acid-containing particles: low nucleic acid-content particles above threshold (LNA-AT) and high nucleic acid-content particles (HNA) likely containing wet-ejected fungal spores and pollen. We find that the daily-average springtime PBAP concentration (1 to 5 mu m diameter) ranged between 1.4 x 10(4) and 1.1 x 10(5) m(-3). The LNA-AT population dominated PBAP during dry days (72 +/- 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA comprised up to 92% of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that fluorescent biological aerosol particles (FBAP) and total FCM counts are similar; HNA (from FCM) moderately correlated with ABC-type FBAP concentrations throughout the sampling period (and for the same particle size range, 1-5 mu m diameter). However, the FCM LNA-AT population, possibly containing bacterial cells, did not correlate with any FBAP type. The lack of correlation of any WIBS FBAP type with the LNA-AT suggests that airborne bacterial cells may be more difficult to unambiguously detect with autofluorescence than currently thought. Identification of bacterial cells even in the FCM (LNA-AT population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed the highest concentration on a humid and warm day after a rain event (14 April 2015), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days and an LNA-AT population dominating the bioaerosol community during dry days. |
领域 | 地球科学 |
收录类别 | SCI-E |
WOS记录号 | WOS:000514165600004 |
WOS关键词 | BIOLOGICAL AEROSOL-PARTICLES ; ICE-NUCLEATION ACTIVITY ; ATMOSPHERIC BIOAEROSOLS ; AIRBORNE OBSERVATIONS ; CLUSTER-ANALYSIS ; FUNGAL SPORES ; POLLEN ; URBAN ; POPULATIONS ; BACTERIA |
WOS类目 | Environmental Sciences ; Meteorology & Atmospheric Sciences |
WOS研究方向 | Environmental Sciences & Ecology ; Meteorology & Atmospheric Sciences |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/278622 |
专题 | 地球科学 |
作者单位 | 1.Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA; 2.Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA; 3.Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA; 4.NASA, Langley Res Ctr, Sci Directorate, Sch Biol Sci,Chem & Dynam Branch, Hampton, VA 23681 USA; 5.Duke Univ, Dept Civil & Environm Engn, Durham, NC 27706 USA; 6.Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA; 7.Fdn Res & Technol Hellas, Inst Chem Engn Sci, Patras 26504, Greece; 8.Ecole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lab Atmospher Proc & Their Impacts LAPI, CH-1015 Lausanne, Switzerland; 9.Puerto Rico Sci Technol & Res Trust, Rio Piedras, PR 00927 USA; 10.Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA |
推荐引用方式 GB/T 7714 | Negron, Arnaldo,DeLeon-Rodriguez, Natasha,Waters, Samantha M.,et al. Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia[J]. ATMOSPHERIC CHEMISTRY AND PHYSICS,2020,20(3):1817-1838. |
APA | Negron, Arnaldo.,DeLeon-Rodriguez, Natasha.,Waters, Samantha M..,Ziemba, Luke D..,Anderson, Bruce.,...&Nenes, Athanasios.(2020).Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia.ATMOSPHERIC CHEMISTRY AND PHYSICS,20(3),1817-1838. |
MLA | Negron, Arnaldo,et al."Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia".ATMOSPHERIC CHEMISTRY AND PHYSICS 20.3(2020):1817-1838. |
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