Mass-resolved electronic circular dichroism ion spectroscopy

doi:10.1126/science.abb1822

GSTDTAP > 气候变化

DOI	10.1126/science.abb1822
	Mass-resolved electronic circular dichroism ion spectroscopy
	Steven Daly; Frédéric Rosu; Valérie Gabelica
	2020-06-26
发表期刊	Science
出版年	2020
英文摘要	Circular dichroism spectroscopy is widely used to distinguish between nonidentical mirror-image molecules. The technique relies on differential absorption of left versus right circularly polarized light and therefore tends to require solution-phase samples for adequate sensitivity. Daly et al. now report gas-phase circular dichroism spectra of DNA oligonucleotides based on detection of photodetached electrons rather than transmitted light (see the Perspective by Barran). The salient spectral features matched those in solution. Pairing the technique with mass spectrometry enables prior mass selection of particular molecules for analysis. Science , this issue p. [1465][1]; see also p. [1426][2] DNA and proteins are chiral: Their three-dimensional structures cannot be superimposed with their mirror images. Circular dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case of mixtures. We recorded the electronic circular dichroism spectra of DNA helices separated in a mass spectrometer. We studied guanine-rich strands having various secondary structures, electrosprayed them as negative ions, irradiated them with an ultraviolet nanosecond optical parametric oscillator laser, and measured the difference in electron photodetachment efficiency between left and right circularly polarized light. The reconstructed circular dichroism ion spectra resembled those of their solution-phase counterparts, thereby allowing us to assign the DNA helical topology. The ability to measure circular dichroism directly on biomolecular ions expands the capabilities of mass spectrometry for structural analysis. [1]: /lookup/doi/10.1126/science.abb1822 [2]: /lookup/doi/10.1126/science.abc1294
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/278213
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Steven Daly,Frédéric Rosu,Valérie Gabelica. Mass-resolved electronic circular dichroism ion spectroscopy[J]. Science,2020.
APA	Steven Daly,Frédéric Rosu,&Valérie Gabelica.(2020).Mass-resolved electronic circular dichroism ion spectroscopy.Science.
MLA	Steven Daly,et al."Mass-resolved electronic circular dichroism ion spectroscopy".Science (2020).