Cryo-EM structure of the human cohesin-NIPBL-DNA complex

doi:10.1126/science.abb0981

GSTDTAP > 气候变化

DOI	10.1126/science.abb0981
	Cryo-EM structure of the human cohesin-NIPBL-DNA complex
	Zhubing Shi; Haishan Gao; Xiao-chen Bai; Hongtao Yu
	2020-06-26
发表期刊	Science
出版年	2020
英文摘要	Cohesin is a multiprotein complex that entraps sister chromatids for chromosome segregation and regulates transcription by extruding DNA loops to shape DNA organization. Shi et al. determined the structure of human cohesin bound to the protein NIBPL, which helps load cohesin onto DNA, and DNA at medium resolution by cryo–electron microscopy. Two adenosine triphosphatase domains play a key role in cohesin function. The structure explains how NIBPL and DNA synergistically activate these domains and gives insight into how DNA is trapped by cohesin. Science , this issue p. [1454][1] As a ring-shaped adenosine triphosphatase (ATPase) machine, cohesin organizes the eukaryotic genome by extruding DNA loops and mediates sister chromatid cohesion by topologically entrapping DNA. How cohesin executes these fundamental DNA transactions is not understood. Using cryo–electron microscopy (cryo-EM), we determined the structure of human cohesin bound to its loader NIPBL and DNA at medium resolution. Cohesin and NIPBL interact extensively and together form a central tunnel to entrap a 72–base pair DNA. NIPBL and DNA promote the engagement of cohesin’s ATPase head domains and ATP binding. The hinge domains of cohesin adopt an “open washer” conformation and dock onto the STAG1 subunit. Our structure explains the synergistic activation of cohesin by NIPBL and DNA and provides insight into DNA entrapment by cohesin. [1]: /lookup/doi/10.1126/science.abb0981
领域	气候变化 ; 资源环境
URL	查看原文
引用统计	被引频次：112[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/278211
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Zhubing Shi,Haishan Gao,Xiao-chen Bai,et al. Cryo-EM structure of the human cohesin-NIPBL-DNA complex[J]. Science,2020.
APA	Zhubing Shi,Haishan Gao,Xiao-chen Bai,&Hongtao Yu.(2020).Cryo-EM structure of the human cohesin-NIPBL-DNA complex.Science.
MLA	Zhubing Shi,et al."Cryo-EM structure of the human cohesin-NIPBL-DNA complex".Science (2020).