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DOI | 10.1038/s41467-020-16428-w |
Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent | |
Kamennaya, Nina A.1,2,5; Geraki, Kalotina3; Scanlan, David J.2; Zubkov, Mikhail, V1,4 | |
2020-05-26 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2020 |
卷号 | 11期号:1 |
文章类型 | Article |
语种 | 英语 |
国家 | England; Scotland; Israel |
英文摘要 | Bacteria acquire phosphate (P-i) by maintaining a periplasmic concentration below environmental levels. We recently described an extracellular P-i buffer which appears to counteract the gradient required for P-i diffusion. Here, we demonstrate that various treatments to outer membrane (OM) constituents do not affect the buffered P-i because bacteria accumulate P-i in the periplasm, from which it can be removed hypo-osmotically. The periplasmic P-i can be gradually imported into the cytoplasm by ATP-powered transport, however, the proton motive force (PMF) is not required to keep P-i in the periplasm. In contrast, the accumulation of P-i into the periplasm across the OM is PMF-dependent and can be enhanced by light energy. Because the conventional mechanism of P-i-specific transport cannot explain P-i accumulation in the periplasm we propose that periplasmic P-i anions pair with chemiosmotic cations of the PMF and millions of accumulated P-i pairs could influence the periplasmic osmolarity of marine bacteria. The ubiquitous oceanic bacteria harbour an external phosphate buffer for modulating phosphate (P-i) uptake. Here, using both oceanic SAR11, Prochlorococcus and Synechococcus strains as a model, the authors show that the P-i buffer accumulation in the periplasm is proton motive force-dependent and can be enhanced by light energy. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000538812100017 |
WOS关键词 | NORTH-ATLANTIC ; MOLECULAR-BASIS ; PHOSPHORUS ; PROCHLOROCOCCUS ; TRANSPORT ; LIGHT ; PROTEIN ; GENOME ; ENUMERATION ; EVOLUTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/271705 |
专题 | 资源环境科学 |
作者单位 | 1.Natl Oceanog Ctr, Southampton SO14 3ZH, Hants, England; 2.Univ Warwick, Sch Life Sci, Gibbet Hill, Coventry CV4 7AL, W Midlands, England; 3.Harwell Sci & Innovat Campus, Diamond Light Source Ltd, Didcot OX11 0DE, Oxon, England; 4.Scottish Marine Inst, Scottish Assoc Marine Sci, Oban PA37 1QA, Argyll, Scotland; 5.Tel Aviv Univ, George S Wise Fac Life Sci, Sch Plant Sci & Food Secur, IL-6997801 Tel Aviv, Israel |
推荐引用方式 GB/T 7714 | Kamennaya, Nina A.,Geraki, Kalotina,Scanlan, David J.,et al. Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent[J]. NATURE COMMUNICATIONS,2020,11(1). |
APA | Kamennaya, Nina A.,Geraki, Kalotina,Scanlan, David J.,&Zubkov, Mikhail, V.(2020).Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent.NATURE COMMUNICATIONS,11(1). |
MLA | Kamennaya, Nina A.,et al."Accumulation of ambient phosphate into the periplasm of marine bacteria is proton motive force dependent".NATURE COMMUNICATIONS 11.1(2020). |
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