GSTDTAP  > 资源环境科学
DOI10.1073/pnas.1918741117
DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness
Bush, Alex1,2; Monk, Wendy A.3; Compson, Zacchaeus G.1,4; Peters, Daniel L.5; Porter, Teresita M.6,7,8; Shokralla, Shadi7,8; Wright, Michael T. G.7,8; Hajibabaei, Mehrdad7,8; Baird, Donald J.1
2020-04-14
发表期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
出版年2020
卷号117期号:15页码:8539-8545
文章类型Article
语种英语
国家Canada; England
英文摘要

The complexity and natural variability of ecosystems present a challenge for reliable detection of change due to anthropogenic influences. This issue is exacerbated by necessary trade-offs that reduce the quality and resolution of survey data for assessments at large scales. The Peace-Athabasca Delta (PAD) is a large inland wetland complex in northern Alberta, Canada. Despite its geographic isolation, the PAD is threatened by encroachment of oil sands mining in the Athabasca watershed and hydroelectric dams in the Peace watershed. Methods capable of reliably detecting changes in ecosystem health are needed to evaluate and manage risks. Between 2011 and 2016, aquatic macroinvertebrates were sampled across a gradient of wetland flood frequency, applying both microscope-based morphological identification and DNA metabarcoding. By using multispecies occupancy models, we demonstrate that DNA metabarcoding detected a much broader range of taxa and more taxa per sample compared to traditional morphological identification and was essential to identifying significant responses to flood and thermal regimes. We show that family-level occupancy masks high variation among genera and quantify the bias of barcoding primers on the probability of detection in a natural community. Interestingly, patterns of community assembly were nearly random, suggesting a strong role of stochasticity in the dynamics of the metacommunity. This variability seriously compromises effective monitoring at local scales but also reflects resilience to hydrological and thermal variability. Nevertheless, simulations showed the greater efficiency of metabarcoding, particularly at a finer taxonomic resolution, provided the statistical power needed to detect change at the landscape scale.


英文关键词occupancy detectability taxonomic resolution stochasticity power analysis
领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000526871700047
WOS关键词FRESH-WATER ECOSYSTEMS ; SPECIES RICHNESS ; TAXONOMIC RESOLUTION ; IMPERFECT DETECTION ; MULTIPLE STRESSORS ; OCCUPANCY ; MODELS ; ACCUMULATION ; CONSERVATION ; PERFORMANCE
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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被引频次:48[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/249720
专题资源环境科学
作者单位1.Univ New Brunswick, Environm & Climate Change Canada, Canadian Rivers Inst, Dept Biol, Fredericton, NB E3B 5A3, Canada;
2.Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YW, England;
3.Univ New Brunswick, Environm & Climate Change Canada, Canadian Rivers Inst, Fac Forestry & Environm Management, Fredericton, NB E3B 5A3, Canada;
4.Ctr Environm Genom Applicat, St John, NF A1A 0R6, Canada;
5.Univ Victoria, Watershed Hydrol & Ecol Res Div, Environm & Climate Change Canada, Victoria, BC V8P 5C2, Canada;
6.Nat Resources Canada, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada;
7.Univ Guelph, Ctr Biodivers Genom, Guelph, ON N1G 2W1, Canada;
8.Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada
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GB/T 7714
Bush, Alex,Monk, Wendy A.,Compson, Zacchaeus G.,et al. DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(15):8539-8545.
APA Bush, Alex.,Monk, Wendy A..,Compson, Zacchaeus G..,Peters, Daniel L..,Porter, Teresita M..,...&Baird, Donald J..(2020).DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(15),8539-8545.
MLA Bush, Alex,et al."DNA metabarcoding reveals metacommunity dynamics in a threatened boreal wetland wilderness".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.15(2020):8539-8545.
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