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DOI | 10.1126/science.aah7111 |
CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells | |
S. John Liu; Max A. Horlbeck; Seung Woo Cho; Harjus S. Birk; Martina Malatesta; Daniel He; Frank J. Attenello; Jacqueline E. Villalta; Min Y. Cho; Yuwen Chen; Mohammad A. Mandegar; Michael P. Olvera; Luke A. Gilbert; Bruce R. Conklin; Howard Y. Chang; Jonathan S. Weissman; Daniel A. Lim | |
2017-01-06 | |
发表期刊 | Science |
出版年 | 2017 |
卷号 | 355期号:6320 |
英文摘要 | AbstractThe human genome produces thousands of long noncoding RNAs (lncRNAs)—transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type–specific manner. These data underscore the functional importance and cell type specificity of many lncRNAs. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/243061 |
专题 | 地球科学 资源环境科学 气候变化 |
推荐引用方式 GB/T 7714 | S. John Liu,Max A. Horlbeck,Seung Woo Cho,et al. CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells[J]. Science,2017,355(6320). |
APA | S. John Liu.,Max A. Horlbeck.,Seung Woo Cho.,Harjus S. Birk.,Martina Malatesta.,...&Daniel A. Lim.(2017).CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells.Science,355(6320). |
MLA | S. John Liu,et al."CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells".Science 355.6320(2017). |
条目包含的文件 | 条目无相关文件。 |
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