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DOI | 10.1289/EHP852 |
Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure | |
Liu, Xiaoyu1,2,3; Li, Meijing1,2,3; Xia, Xian1,2; Li, Xueming1,2,3; Chen, Zhucheng1,2 | |
2017-04-27 | |
发表期刊 | NATURE |
ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 544期号:7651页码:440-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | Chromatin remodellers are helicase-like, ATP-dependent enzymes that alter chromatin structure and nucleosome positions to allow regulatory proteins access to DNA. Here we report the cryo-electron microscopy structure of chromatin remodeller Switch/sucrose non-fermentable (SWI2/SNF2) from Saccharomyces cerevisiae bound to the nucleosome. The structure shows that the two core domains of Snf2 are realigned upon nucleosome binding, suggesting activation of the enzyme. The core domains contact each other through two induced Brace helices, which are crucial for coupling ATP hydrolysis to chromatin remodelling. Snf2 binds to the phosphate backbones of one DNA gyre of the nucleosome mainly through its helicase motifs within the major domain cleft, suggesting a conserved mechanism of substrate engagement across different remodellers. Snf2 contacts the second DNA gyre via a positively charged surface, providing a mechanism to anchor the remodeller at a fixed position of the nucleosome. Snf2 locally deforms nucleosomal DNA at the site of binding, priming the substrate for the remodelling reaction. Together, these findings provide mechanistic insights into chromatin remodelling. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000400051900035 |
WOS关键词 | NUCLEOSOME CORE PARTICLE ; ELECTRON-MICROSCOPY ; DNA TRANSLOCATION ; CRYO-EM ; COMPLEX ; SWI/SNF ; ISWI ; VISUALIZATION ; ARCHITECTURE ; RESOLUTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/23275 |
专题 | 资源环境科学 |
作者单位 | 1.Tsinghua Univ, Minist Educ, Key Lab Prot Sci, Beijing 100084, Peoples R China; 2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China; 3.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Xiaoyu,Li, Meijing,Xia, Xian,et al. Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure[J]. NATURE,2017,544(7651):440-+. |
APA | Liu, Xiaoyu,Li, Meijing,Xia, Xian,Li, Xueming,&Chen, Zhucheng.(2017).Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure.NATURE,544(7651),440-+. |
MLA | Liu, Xiaoyu,et al."Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure".NATURE 544.7651(2017):440-+. |
条目包含的文件 | 条目无相关文件。 |
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