Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aav1741 |
The biochemical basis of microRNA targeting efficacy | |
McGeary, Sean E.1,2,3; Lin, Kathy S.1,2,3,4; Shi, Charlie Y.1,2,3; Pham, Thy M.1,2,3; Bisaria, Namita1,2,3; Kelley, Gina M.1,2,3; Bartel, David P.1,2,3,4 | |
2019-12-20 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6472页码:1470-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | MicroRNAs (miRNAs) act within Argonaute proteins to guide repression of messenger RNA targets. Although various approaches have provided insight into target recognition, the sparsity of miRNA-target affinity measurements has limited understanding and prediction of targeting efficacy. Here, we adapted RNA bind-n-seq to enable measurement of relative binding affinities between Argonaute-miRNA complexes and all sequences <= 12 nucleotides in length. This approach revealed noncanonical target sites specific to each miRNA, miRNA-specific differences in canonical target-site affinities, and a 100-fold impact of dinucleotides flanking each site. These data enabled construction of a biochemical model of miRNA-mediated repression, which was extended to all miRNA sequences using a convolutional neural network. This model substantially improved prediction of cellular repression, thereby providing a biochemical basis for quantitatively integrating miRNAs into gene-regulatory networks. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000503861000044 |
WOS关键词 | WIDE IDENTIFICATION ; RNA ; MIRNA ; REVEALS ; SEQUENCE ; DETERMINANTS ; SPECIFICITY ; PROTEIN ; IMPACT ; SITES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226498 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Whitehead Inst Biomed Res, Howard Hughes Med Inst, 9 Cambridge Ctr, Cambridge, MA 02142 USA; 2.Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA; 3.MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 4.MIT, Computat & Syst Biol Program, 77 Massachusetts Ave, Cambridge, MA 02139 USA |
推荐引用方式 GB/T 7714 | McGeary, Sean E.,Lin, Kathy S.,Shi, Charlie Y.,et al. The biochemical basis of microRNA targeting efficacy[J]. SCIENCE,2019,366(6472):1470-+. |
APA | McGeary, Sean E..,Lin, Kathy S..,Shi, Charlie Y..,Pham, Thy M..,Bisaria, Namita.,...&Bartel, David P..(2019).The biochemical basis of microRNA targeting efficacy.SCIENCE,366(6472),1470-+. |
MLA | McGeary, Sean E.,et al."The biochemical basis of microRNA targeting efficacy".SCIENCE 366.6472(2019):1470-+. |
条目包含的文件 | 条目无相关文件。 |
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