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DOI | 10.1126/science.aax8055 |
Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types | |
Lee, Donghoon; Kume, Maiko; Holy, Timothy E. | |
2019-12-13 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6471页码:1384-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Neural circuit analysis relies on having molecular markers for specific cell types. However, for a cell type identified only by its circuit function, the process of identifying markers remains laborious. We developed physiological optical tagging sequencing (PhOTseq), a technique for tagging and expression profiling of cells on the basis of their functional properties. PhOTseq was capable of selecting rare cell types and enriching them by nearly 100-fold. We applied PhOTseq to the challenge of mapping receptor-ligand pairings among pheromone-sensing neurons in mice. Together with in vivo ectopic expression of vomeronasal chemoreceptors, PhOTseq identified the complete combinatorial receptor code for a specific set of ligands. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000502802300061 |
WOS关键词 | ODORANT RECEPTORS ; VOMERONASAL ; ORGANIZATION ; EXPRESSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226450 |
专题 | 环境与发展全球科技态势 |
作者单位 | Washington Univ, Dept Neurosci, Sch Med, St Louis, MO 63130 USA |
推荐引用方式 GB/T 7714 | Lee, Donghoon,Kume, Maiko,Holy, Timothy E.. Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types[J]. SCIENCE,2019,366(6471):1384-+. |
APA | Lee, Donghoon,Kume, Maiko,&Holy, Timothy E..(2019).Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types.SCIENCE,366(6471),1384-+. |
MLA | Lee, Donghoon,et al."Sensory coding mechanisms revealed by optical tagging of physiologically defined neuronal types".SCIENCE 366.6471(2019):1384-+. |
条目包含的文件 | 条目无相关文件。 |
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