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DOI | 10.1126/science.aaz3418 |
DNA loop extrusion by human cohesin | |
Davidson, Iain F.1; Bauer, Benedikt1; Goetz, Daniela1,2; Tang, Wen1; Wutz, Gordana1; Peters, Jan-Michael1 | |
2019-12-13 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6471页码:1338-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Austria; Germany |
英文摘要 | Eukaryotic genomes are folded into loops and topologically associating domains, which contribute to chromatin structure, gene regulation, and gene recombination. These structures depend on cohesin, a ring-shaped DNA- entrapping adenosine triphosphatase (ATPase) complex that has been proposed to form loops by extrusion. Such an activity has been observed for condensin, which forms loops in mitosis, but not for cohesin. Using biochemical reconstitution, we found that single human cohesin complexes form DNA loops symmetrically at rates up to 2.1 kilo-base pairs per second. Loop formation and maintenance depend on cohesin's ATPase activity and on NIPBL-MAU2, but not on topological entrapment of DNA by cohesin. During loop formation, cohesin and NIPBL-MAU2 reside at the base of loops, which indicates that they generate loops by extrusion. Our results show that cohesin and NIPBL-MAU2 form an active holoenzyme that interacts with DNA either pseudo-topologically or non-topologically to extrude genomic interphase DNA into loops. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000502802300051 |
WOS关键词 | MAMMALIAN GENOMES ; SCC2 ; COMPLEXES ; CONDENSIN ; DOMAINS ; BINDING ; CTCF ; WAPL ; ORGANIZATION ; MECHANISM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226440 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Vienna BioCtr VBC, Res Inst Mol Pathol IMP, A-1030 Vienna, Austria; 2.AMS Adv Med Serv GmbH, D-6816 Mannheim, Germany |
推荐引用方式 GB/T 7714 | Davidson, Iain F.,Bauer, Benedikt,Goetz, Daniela,et al. DNA loop extrusion by human cohesin[J]. SCIENCE,2019,366(6471):1338-+. |
APA | Davidson, Iain F.,Bauer, Benedikt,Goetz, Daniela,Tang, Wen,Wutz, Gordana,&Peters, Jan-Michael.(2019).DNA loop extrusion by human cohesin.SCIENCE,366(6471),1338-+. |
MLA | Davidson, Iain F.,et al."DNA loop extrusion by human cohesin".SCIENCE 366.6471(2019):1338-+. |
条目包含的文件 | 条目无相关文件。 |
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