Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aaw8208 |
Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway | |
Choi, Hyun-Kyu1,2,3; Min, Duyoung4,5; Kang, Hyunook2; Shon, Min Ju2,3; Rah, Sang-Hyun1,2,3; Kim, Hak Chan2; Jeong, Hawoong1; Choi, Hee-Jung2; Bowie, James U.4; Yoon, Tae-Young2,3 | |
2019-11-29 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6469页码:1150-+ |
文章类型 | Article |
语种 | 英语 |
国家 | South Korea; USA |
英文摘要 | To understand membrane protein biogenesis, we need to explore folding within a bilayer context. Here, we describe a single-molecule force microscopy technique that monitors the folding of helical membrane proteins in vesicle and bicelle environments. After completely unfolding the protein at high force, we lower the force to initiate folding while transmembrane helices are aligned in a zigzag manner within the bilayer, thereby imposing minimal constraints on folding. We used the approach to characterize the folding pathways of the Escherichia coli rhomboid protease GlpG and the human beta(2)-adrenergic receptor. Despite their evolutionary distance, both proteins fold in a strict N-to-C-terminal fashion, accruing structures in units of helical hairpins. These common features suggest that integral helical membrane proteins have evolved to maximize their fitness with cotranslational folding. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000500039200047 |
WOS关键词 | FLUCTUATION THEOREM ; CRYSTAL-STRUCTURE ; ENERGY LANDSCAPE ; INFORMATION ; DIFFUSION ; INSERTION ; KINETICS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226326 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Korea Adv Inst Sci & Technol, Dept Phys, Daejeon 34141, South Korea; 2.Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea; 3.Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 08826, South Korea; 4.Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA; 5.Ulsan Natl Inst Sci & Technol, Dept Chem, Ulsan 44919, South Korea |
推荐引用方式 GB/T 7714 | Choi, Hyun-Kyu,Min, Duyoung,Kang, Hyunook,et al. Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway[J]. SCIENCE,2019,366(6469):1150-+. |
APA | Choi, Hyun-Kyu.,Min, Duyoung.,Kang, Hyunook.,Shon, Min Ju.,Rah, Sang-Hyun.,...&Yoon, Tae-Young.(2019).Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway.SCIENCE,366(6469),1150-+. |
MLA | Choi, Hyun-Kyu,et al."Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway".SCIENCE 366.6469(2019):1150-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论