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DOI | 10.1126/science.aaw5754 |
Dissecting primate early post-implantation development using long-term in vitro embryo culture | |
Niu, Yuyu1; Sun, Nianqin1; Li, Chang1; Lei, Ying2; Huang, Zhihao2,3; Wu, Jun4,5,6; Si, Chenyang1; Dai, Xi2,3; Liu, Chuanyu2,3; Wei, Jingkuan1; Liu, Longqi2,3; Feng, Su7; Kang, Yu1; Si, Wei1; Wang, Hong1; Zhang, E.1; Zhao, Lu1; Li, Ziwei1; Luo, Xi8; Cui, Guizhong9; Peng, Guangdun8,9; Belmonte, Juan Carlos Izpisua; Ji, Weizhi1,10; Tan, Tao1 | |
2019-11-15 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6467页码:837-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; USA |
英文摘要 | The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms that underlie this transition. We established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bilaminar disc formation, amniotic and yolk sac cavitation, and primordial germ cell-like cell (PGCLC) differentiation. Single-cell RNA-sequencing analysis revealed development trajectories of primitive endoderm, trophectoderm, epiblast lineages, and PGCLCs. Analysis of single-cell chromatin accessibility identified transcription factors specifying each cell type. Our results reveal critical developmental events and complex molecularmechanisms underlying nonhuman primate embryogenesis in the early postimplantation period, with possible relevance to human development. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000496946200046 |
WOS关键词 | CELL FATE DECISIONS ; STEM-CELLS ; SELF-ORGANIZATION ; READ ALIGNMENT ; RNA-SEQ ; MOUSE ; DIFFERENTIATION ; MONKEY ; EMBRYOGENESIS ; ACCESSIBILITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226230 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Inst Primate Translat Med, Yunnan Key Lab Primate Biomed Res, Kunming 650500, Yunnan, Peoples R China; 2.BGI Shenzhen, Shenzhen 518083, Guangdong, Peoples R China; 3.BGI Shenzhen, China Natl GeneBank, Shenzhen 518120, Guangdong, Peoples R China; 4.Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA; 5.Univ Texas Southwestern Med Ctr Dallas, Hamon Ctr Regenerat Sci & Med, Dallas, TX 75390 USA; 6.Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 USA; 7.Chinese Acad Sci, State Key Lab Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200032, Peoples R China; 8.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, CAS Key Lab Regenerat Biol, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou 510530, Guangdong, Peoples R China; 9.Guangzhou Regenerat Med & Hlth Guangdong Lab GRMH, Ctr Cell Lineage & Atlas, Guangzhou 510530, Guangdong, Peoples R China; 10.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai 200032, Peoples R China |
推荐引用方式 GB/T 7714 | Niu, Yuyu,Sun, Nianqin,Li, Chang,et al. Dissecting primate early post-implantation development using long-term in vitro embryo culture[J]. SCIENCE,2019,366(6467):837-+. |
APA | Niu, Yuyu.,Sun, Nianqin.,Li, Chang.,Lei, Ying.,Huang, Zhihao.,...&Tan, Tao.(2019).Dissecting primate early post-implantation development using long-term in vitro embryo culture.SCIENCE,366(6467),837-+. |
MLA | Niu, Yuyu,et al."Dissecting primate early post-implantation development using long-term in vitro embryo culture".SCIENCE 366.6467(2019):837-+. |
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