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DOI | 10.1126/science.aav7793 |
Spontaneous emergence of cell-like organization in Xenopus egg extracts | |
Cheng, Xianrui1; Ferrell, James E., Jr.1,2 | |
2019-11-01 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6465页码:631-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Every daughter cell inherits two things from its mother: genetic information and a spatially organized complement of macromolecular complexes and organelles. The extent to which de novo selforganization, as opposed to inheritance of an already organized state, can suffice to yield functional cells is uncertain. We used Xenopus laevis egg extracts to show that homogenized interphase egg cytoplasm self-organizes over the course of similar to 30 minutes into compartments 300 to 400 micrometers in length that resemble cells. Formation of these cell-like compartments required adenosine triphosphate and microtubule polymerization but did not require added demembranated sperm nuclei with their accompanying centrosomes or actin polymerization. In cycling extracts with added sperm, the compartments underwent multiple cycles of division and reorganization, with mother compartments giving rise to two daughters at the end of each mitotic cycle. These results indicate that the cytoplasm can generate much of the spatial organization and cell cycle function of the early embryo. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000494465700048 |
WOS关键词 | SELF-ORGANIZATION ; RECONSTITUTION ; MICROTUBULES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226133 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Stanford Univ, Sch Med, Dept Chem & Syst Biol, Stanford, CA 94305 USA; 2.Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA |
推荐引用方式 GB/T 7714 | Cheng, Xianrui,Ferrell, James E., Jr.. Spontaneous emergence of cell-like organization in Xenopus egg extracts[J]. SCIENCE,2019,366(6465):631-+. |
APA | Cheng, Xianrui,&Ferrell, James E., Jr..(2019).Spontaneous emergence of cell-like organization in Xenopus egg extracts.SCIENCE,366(6465),631-+. |
MLA | Cheng, Xianrui,et al."Spontaneous emergence of cell-like organization in Xenopus egg extracts".SCIENCE 366.6465(2019):631-+. |
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