Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP2387 |
Hydroxylated Polybrominated Biphenyl Ethers Exert Estrogenic Effects via Non-Genomic G Protein-Coupled Estrogen Receptor Mediated Pathways | |
Cao, Lin-Ying1,2; Ren, Xiao-Min1; Yang, Yu1; Wan, Bin1; Guo, Liang-Hong1,2,3; Chen, De3; Fan, Yong3 | |
2018-05-01 | |
发表期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES |
ISSN | 0091-6765 |
EISSN | 1552-9924 |
出版年 | 2018 |
卷号 | 126期号:5 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | BACKGROUND: Numerous studies have indicated the estrogenic effects of polybrominated diphenyl ethers (PBDEs) and hydroxylated PBDEs (OH-PBDEs). However, the previous mechanistic studies focused on their estrogenic effects through genomic transcriptional activation of estrogen receptors. OBJECTIVE: The present study aimed to investigate the estrogenic effects of PBDEs and OH-PBDEs via nongenomic G protein-coupled estrogen receptor (GPER) pathways. METHODS: The binding affinities of 12 PBDEs and 18 OH-PBDEs with GPER were determined by a fluorescence competitive binding assay in a human breast cancer cell line (SKBR3). Molecular docking was performed to simulate the interactions. Their activities on GPER pathways were investigated by detecting calcium mobilization and cyclic adenosine monophosphate (cAMP) accumulation in SKBR3 cells. The effects on SKBR3 cell migration were investigated using Boyden chamber and wound-healing assays. RESULTS: Our results showed that 11 of the OH-PBDEs but none of the PBDEs hound to GPER directly. Relative binding affinities ranged from 1.3% to 20.0% compared to 17 beta-estradiol. Docking results suggested that the hydroxyl group played an essential role in the binding of OH-PBDEs to GPER by forming hydrogen bond interactions. Most of the OH-PBDEs activated subsequent GPER signaling pathways. Among them, 4'-OH-BDE-049, 5'-OH-BDE-099, and 3'-OH-BDE-154 displayed the highest activity with lowest effective concentrations (LOECs) of 10-100 nM. These three OH-PBDEs also promoted SKBR3 cell migration via GPER pathways with LOECs of 0.1-1 mu M. CONCLUSION: OH-PBDEs could bind to GPER, activate the subsequent signaling pathways, and promote SKBR3 cell migration via GPER pathways. OH-PBDEs might exert estrogenic effects by a novel nongenomic mechanism involving the activation of GPER at nanomolar concentrations. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000432619700002 |
WOS关键词 | BREAST-CANCER CELLS ; GENE-EXPRESSION CHANGES ; DIPHENYL ETHERS ; BISPHENOL-A ; DEVELOPMENTAL EXPOSURE ; ENDOCRINE DISRUPTION ; MIXTURE DE-71 ; IN-VITRO ; GPR30 ; GPER |
WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health ; Toxicology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/22579 |
专题 | 资源环境科学 |
作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, 18 Shuangqing Rd,POB 2871, Beijing 100085, Peoples R China; 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China; 3.Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstet Dis Guangdong Prov, Key Lab Reprod & Genet,Guangdong Higher Educ Inst, Guangzhou, Guangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Cao, Lin-Ying,Ren, Xiao-Min,Yang, Yu,et al. Hydroxylated Polybrominated Biphenyl Ethers Exert Estrogenic Effects via Non-Genomic G Protein-Coupled Estrogen Receptor Mediated Pathways[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2018,126(5). |
APA | Cao, Lin-Ying.,Ren, Xiao-Min.,Yang, Yu.,Wan, Bin.,Guo, Liang-Hong.,...&Fan, Yong.(2018).Hydroxylated Polybrominated Biphenyl Ethers Exert Estrogenic Effects via Non-Genomic G Protein-Coupled Estrogen Receptor Mediated Pathways.ENVIRONMENTAL HEALTH PERSPECTIVES,126(5). |
MLA | Cao, Lin-Ying,et al."Hydroxylated Polybrominated Biphenyl Ethers Exert Estrogenic Effects via Non-Genomic G Protein-Coupled Estrogen Receptor Mediated Pathways".ENVIRONMENTAL HEALTH PERSPECTIVES 126.5(2018). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论