Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP4945 |
Global Transcriptional Analysis of Nontransformed Human Intestinal Epithelial Cells (FHs 74 Int) after Exposure to Selected Drinking Water Disinfection By-Products | |
Prochazka, Erik1; Melvin, Steven D.1; Escher, Beate I.1,2,3; Plewa, Michael J.4,5; Leusch, Frederic D. L.1 | |
2019-11-01 | |
发表期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES
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ISSN | 0091-6765 |
EISSN | 1552-9924 |
出版年 | 2019 |
卷号 | 127期号:11 |
文章类型 | Article |
语种 | 英语 |
国家 | Australia; Germany; USA |
英文摘要 | BACKGROUND: Drinking water disinfection inadvertently leads to the formation of numerous disinfection by-products (DBPs), sonic of which are cytotoxic, mutagenic, genotoxic, teratogenic, and potential carcinogens both in vitro and in vivo. OBJECTIVES: We investigated alterations to global gene expression (GE) in nontransformed human small intestine epithelial cells FHs 74 Int) after exposure to six brominated and two chlorinated DBPs: bromoacetic acid (BAA), hrornoacetonitrile (BAN), 2,6-dihromo-p-benzoquinone (DBBQ), bromoacetamide (BAM), tribromoacetaldehyde (TWAT.), bromate (BrO; trichloroacetic acid (TCAA), and trichloroaceffildehyde (TCAL). METHODS: Using whole-genome cDNA microarray technology (Illumina), we examined GE in nontransformed human cells after 4 h exposure to DBPs at predetermined equipotent concentrations, identified significant changes in gene expression (p < 0.01), and investigated the relevance of these genes to specific toxicity pathways via gene and pathway enrichment analysis. RESULTS: Genes related to activation of oxidative stress responsive pathways exhibited fewer alterations than expected based on prior work, whereas all DBPs induced notable effects on transcription of genes related to immunity and inflammation. DISCUSSION: Our results suggest that alterations to genes associated with immune and inflammatory pathways play an important role in the potential adverse health effects of exposure to DBPs. The interrelationship between these pathways and the production of reactive oxygen species (ROS) may explain the common occurrence of oxidative stress in other studies exploring DBP toxicity. Finally, transcriptional changes and shared induction of toxicity pathways observed for all DBPs caution of additive effects of mixtures and suggest further assessment of adverse health effects of mixtures is warranted. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000500762600007 |
WOS关键词 | TOLL-LIKE RECEPTORS ; OXIDATIVE STRESS ; TOXICOGENOMIC ANALYSIS ; CHRONIC INFLAMMATION ; HALOACETIC ACID ; BLADDER-CANCER ; CHEMICAL CONTAMINANTS ; TOXICITY ; GENOTOXICITY ; CYTOTOXICITY |
WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health ; Toxicology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/224617 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Griffith Univ, Australian Rivers Inst, Sch Environm & Sci, Gold Coast, Qld, Australia; 2.Helmholtz Ctr Environm Res UEZ, Dept Cell Toxicol, Leipzig, Germany; 3.Eberhard Karls Univ Tubingen, Ctr Appl Geosci, Environm Toxicol, Tubingen, Germany; 4.Univ Illinois, Dept Crop Sci, Urbana, IL USA; 5.Univ Illinois, Safe Global Water Inst, Urbana, IL USA |
推荐引用方式 GB/T 7714 | Prochazka, Erik,Melvin, Steven D.,Escher, Beate I.,et al. Global Transcriptional Analysis of Nontransformed Human Intestinal Epithelial Cells (FHs 74 Int) after Exposure to Selected Drinking Water Disinfection By-Products[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2019,127(11). |
APA | Prochazka, Erik,Melvin, Steven D.,Escher, Beate I.,Plewa, Michael J.,&Leusch, Frederic D. L..(2019).Global Transcriptional Analysis of Nontransformed Human Intestinal Epithelial Cells (FHs 74 Int) after Exposure to Selected Drinking Water Disinfection By-Products.ENVIRONMENTAL HEALTH PERSPECTIVES,127(11). |
MLA | Prochazka, Erik,et al."Global Transcriptional Analysis of Nontransformed Human Intestinal Epithelial Cells (FHs 74 Int) after Exposure to Selected Drinking Water Disinfection By-Products".ENVIRONMENTAL HEALTH PERSPECTIVES 127.11(2019). |
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