Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP876 |
Effects of Coal Fly Ash Particulate Matter on the Antimicrobial Activity of Airway Surface Liquid | |
Buonfiglio, Luis G. Vargas1; Mudunkotuwa, Imali A.2; Abou Alaiwa, Mahmoud H.1; Calderon, Oriana G. Vanegas3; Borcherding, Jennifer A.1; Gerke, Alicia K.1; Zabner, Joseph1; Grassian, Vicki H.4,5,6; Comellas, Alejandro P.1 | |
2017-07-01 | |
发表期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES |
ISSN | 0091-6765 |
EISSN | 1552-9924 |
出版年 | 2017 |
卷号 | 125期号:7 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | BACKGROUND: Sustained exposure to ambient particulate matter (PM) is a global cause of mortality. Coal fly ash (CFA) is a byproduct of coal combustion and is a source of anthropogenic PM with worldwide health relevance. The airway epithelia are lined with fluid called airway surface liquid (ASL), which contains antimicrobial proteins and peptides (AMPs). Cationic AMPs bind negatively charged bacteria to exert their antimicrobial activity. PM arriving in the airways could potentially interact with AMPs in the ASL to affect their antimicrobial activity. OBJECTIVES: We hypothesized that PM can interact with ASL AMPs to impair their antimicrobial activity. METHODS: We exposed pig and human airway explants, pig and human ASL, and the human cationic AMPs beta-defensin-3, LL-37, and lysozyme to CFA or control. Thereafter, we assessed the antimicrobial activity of exposed airway samples using both bioluminescence and standard colony forming unit assays. We investigated PM AMP electrostatic interaction by attenuated total reflection Fourier-transform infrared spectroscopy and measuring the zeta potential. We also studied the adsorption of AMPs on PM. RESULTS: We found increased bacterial survival in CFA-exposed airway explants, ASL, and AMPs. In addition, we report that PM with a negative surface charge can adsorb cationic AMPs and form negative particle-protein complexes. CONCLUSION: We propose that when CFA arrives at the airway, it rapidly adsorbs AMPs and creates negative complexes, thereby decreasing the functional amount of AMPs capable of killing pathogens. These results provide a novel translational insight into an early mechanism for how ambient PM increases the susceptibility of the airways to bacterial infection. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000413791300011 |
WOS关键词 | STAPHYLOCOCCUS-AUREUS ; CYSTIC-FIBROSIS ; ATMOSPHERIC DUST ; INNATE IMMUNITY ; POLLUTION ; COLONIZATION ; PNEUMONIA ; HEALTH ; CHINA ; GENE |
WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health ; Toxicology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/22231 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Iowa, Dept Internal Med, Roy J & Lucille A Carver Coll Med, Iowa City, IA 52242 USA; 2.Univ Iowa, Dept Chem, Coll Liberal Arts & Sci, Iowa City, IA 52242 USA; 3.Univ Iowa, Dept Pediat, Roy J & Lucille A Carver Coll Med, Iowa City, IA 52242 USA; 4.Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA; 5.Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA; 6.Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Buonfiglio, Luis G. Vargas,Mudunkotuwa, Imali A.,Abou Alaiwa, Mahmoud H.,et al. Effects of Coal Fly Ash Particulate Matter on the Antimicrobial Activity of Airway Surface Liquid[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2017,125(7). |
APA | Buonfiglio, Luis G. Vargas.,Mudunkotuwa, Imali A..,Abou Alaiwa, Mahmoud H..,Calderon, Oriana G. Vanegas.,Borcherding, Jennifer A..,...&Comellas, Alejandro P..(2017).Effects of Coal Fly Ash Particulate Matter on the Antimicrobial Activity of Airway Surface Liquid.ENVIRONMENTAL HEALTH PERSPECTIVES,125(7). |
MLA | Buonfiglio, Luis G. Vargas,et al."Effects of Coal Fly Ash Particulate Matter on the Antimicrobial Activity of Airway Surface Liquid".ENVIRONMENTAL HEALTH PERSPECTIVES 125.7(2017). |
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