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DOI | 10.1002/2016WR019254 |
Structural basis for gating the high-conductance Ca2+-activated K+ channel | |
Hite, Richard K.1,2; Tao, Xiao1,2; MacKinnon, Roderick1,2 | |
2017-01-05 | |
发表期刊 | NATURE |
ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 541期号:7635页码:52-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The precise control of an ion channel gate by environmental stimuli is crucial for the fulfilment of its biological role. The gate in Slo1 K+ channels is regulated by two separate stimuli, intracellular Ca2+ concentration and membrane voltage. Slo1 is thus central to understanding the relationship between intracellular Ca2+ and membrane excitability. Here we present the Slo1 structure from Aplysia californica in the absence of Ca2+ and compare it with the Ca2+-bound channel. We show that Ca2+ binding at two unique binding sites per subunit stabilizes an expanded conformation of the Ca2+ sensor gating ring. These conformational changes are propagated from the gating ring to the pore through covalent linkers and through protein interfaces formed between the gating ring and the voltage sensors. The gating ring and the voltage sensors are directly connected through these interfaces, which allow membrane voltage to regulate gating of the pore by influencing the Ca2+ sensors. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000396119500027 |
WOS关键词 | ACTIVATED POTASSIUM CHANNELS ; PLANAR LIPID BILAYERS ; BK CHANNELS ; SKELETAL-MUSCLE ; VOLTAGE SENSOR ; ELECTRON CRYOMICROSCOPY ; QUATERNARY AMMONIUM ; CA-2&-ACTIVATED K ; KINETIC STRUCTURE ; CA2+ BINDING |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/21590 |
专题 | 资源环境科学 |
作者单位 | 1.Rockefeller Univ, 1230 York Ave, New York, NY 10065 USA; 2.Howard Hughes Med Inst, 1230 York Ave, New York, NY 10065 USA |
推荐引用方式 GB/T 7714 | Hite, Richard K.,Tao, Xiao,MacKinnon, Roderick. Structural basis for gating the high-conductance Ca2+-activated K+ channel[J]. NATURE,2017,541(7635):52-+. |
APA | Hite, Richard K.,Tao, Xiao,&MacKinnon, Roderick.(2017).Structural basis for gating the high-conductance Ca2+-activated K+ channel.NATURE,541(7635),52-+. |
MLA | Hite, Richard K.,et al."Structural basis for gating the high-conductance Ca2+-activated K+ channel".NATURE 541.7635(2017):52-+. |
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