Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1073/pnas.1901856116 |
Nitrogen sourcing during viral infection of marine cyanobacteria | |
Waldbauer, Jacob R.; Coleman, Maureen L.; Rizzo, Adriana, I; Campbell, Kathryn L.; Lotus, John; Zhang, Lichun | |
2019 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
出版年 | 2019 |
卷号 | 116期号:31页码:15590-15595 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The building blocks of a virus derived from de novo biosynthesis during infection and/or catabolism of preexisting host cell biomass, and the relative contribution of these 2 sources has important consequences for understanding viral biogeochemistry. We determined the uptake of extracellular nitrogen (N) and its biosynthetic incorporation into both virus and host proteins using an isotope-labeling proteomics approach in a model marine cyanobacterium Synechococcus WH8102 infected by a lytic cyanophage S-SM1. By supplying dissolved N as N-15 postinfection, we found that proteins in progeny phage particles were composed of up to 41% extracellularly derived N, while proteins of the infected host cell showed almost no isotope incorporation, demonstrating that de novo amino acid synthesis continues during infection and contributes specifically and substantially to phage replication. The source of N for phage protein synthesis shifted over the course of infection from mostly host derived in the early stages to more medium derived later on. We show that the photosystem II reaction center proteins D1 and D2, which are auxiliary metabolic genes (AMGs) in the S-SM1 genome, are made de novo during infection in an apparently light-dependent manner. We also identified a small set of host proteins that continue to be produced during infection; the majority are homologs of AMGs in S-SM1 or other viruses, suggesting selective continuation of host protein production during infection. The continued acquisition of nutrients by the infected cell and their utilization for phage replication are significant for both evolution and biogeochemical impact of viruses. |
英文关键词 | biogeochemistry proteomics bacteriophage |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000477812400044 |
WOS关键词 | HOST-VIRUS INTERACTIONS ; PHOTOSYNTHESIS GENES ; ELEMENTAL COMPOSITION ; SYNECHOCOCCUS ; PROCHLOROCOCCUS ; REPRODUCTION ; ORIGIN ; LIGHT ; ACIDS ; DNA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/205154 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | Univ Chicago, Dept Geophys Sci, 5734 S Ellis Ave, Chicago, IL 60637 USA |
推荐引用方式 GB/T 7714 | Waldbauer, Jacob R.,Coleman, Maureen L.,Rizzo, Adriana, I,et al. Nitrogen sourcing during viral infection of marine cyanobacteria[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(31):15590-15595. |
APA | Waldbauer, Jacob R.,Coleman, Maureen L.,Rizzo, Adriana, I,Campbell, Kathryn L.,Lotus, John,&Zhang, Lichun.(2019).Nitrogen sourcing during viral infection of marine cyanobacteria.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(31),15590-15595. |
MLA | Waldbauer, Jacob R.,et al."Nitrogen sourcing during viral infection of marine cyanobacteria".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.31(2019):15590-15595. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论