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DOI | 10.1073/pnas.1803636115 |
Multiple prebiotic metals mediate translation | |
Bray, Marcus S.1; Lenz, Timothy K.2; Haynes, Jay William2; Bowman, Jessica C.2; Petrov, Anton S.2; Reddi, Amit R.2; Hud, Nicholas V.2; Williams, Loren Dean2; Glass, Jennifer B.3 | |
2018-11-27 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
出版年 | 2018 |
卷号 | 115期号:48页码:12164-12169 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Today, Mg2+ is an essential cofactor with diverse structural and functional roles in life's oldest macromolecular machine, the translation system. We tested whether ancient Earth conditions (low O-2, high Fe2+, and high Mn2+) can revert the ribosome to a functional ancestral state. First, SHAPE (selective 2'-hydroxyl acylation analyzed by primer extension) was used to compare the effect of Mg2+, Fe2+, and Mn2+ on the tertiary structure of rRNA. Then, we used in vitro translation reactions to test whether Fe2+ or Mn2+ could mediate protein production, and quantified ribosomal metal content. We found that (i) Mg2+, Fe2+, and Mn2+ had strikingly similar effects on rRNA folding; (ii) Fe2+ and Mn2+ can replace Mg2+ as the dominant divalent cation during translation of mRNA to functional protein; and (iii) Fe and Mn associate extensively with the ribosome. Given that the translation system originated and matured when Fe2+ and Mn2+ were abundant, these findings suggest that Fe2+ and Mn2+ played a role in early ribosomal evolution. |
英文关键词 | translation ribosome iron manganese magnesium |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000451351000050 |
WOS关键词 | RIBOSOMAL-RNA FOLDS ; MAGNESIUM-BINDING ; MANGANESE IONS ; STABILITY ; MECHANISM ; KINASE ; ACID ; AMINOACYLATION ; EVOLUTION ; CATALYSIS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/205019 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Georgia Inst Technol, Sch Biol Sci, Atlanta, GA 30332 USA; 2.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA; 3.Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA |
推荐引用方式 GB/T 7714 | Bray, Marcus S.,Lenz, Timothy K.,Haynes, Jay William,et al. Multiple prebiotic metals mediate translation[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(48):12164-12169. |
APA | Bray, Marcus S..,Lenz, Timothy K..,Haynes, Jay William.,Bowman, Jessica C..,Petrov, Anton S..,...&Glass, Jennifer B..(2018).Multiple prebiotic metals mediate translation.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(48),12164-12169. |
MLA | Bray, Marcus S.,et al."Multiple prebiotic metals mediate translation".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.48(2018):12164-12169. |
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