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DOI | 10.1073/pnas.1804139115 |
Native-state imaging of calcifying and noncalcifying microalgae reveals similarities in their calcium storage organelles | |
Gal, Assaf1,2,6; Sorrentino, Andrea3; Kahil, Keren4; Pereiro, Eva3; Faivre, Damien2,5; Scheffel, Andre1 | |
2018-10-23 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
出版年 | 2018 |
卷号 | 115期号:43页码:11000-11005 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Spain; Israel; France |
英文摘要 | Calcium storage organelles are common to all eukaryotic organisms and play a pivotal role in calcium signaling and cellular calcium homeostasis. In most organelles, the intraorganellar calcium concentrations rarely exceed micromolar levels. Acidic organelles called acidocalcisomes, which concentrate calcium into dense phases together with polyphosphates, are an exception. These organelles have been identified in diverse organisms, but, to date, only in cells that do not form calcium biominerals. Recently, a compartment storing molar levels of calcium together with phosphorous was discovered in an intracellularly calcifying alga, the coccolithophore Emiliania huxleyi, raising a possible connection between calcium storage organelles and calcite biomineralization. Here we used cryoimaging and cryospectroscopy techniques to investigate the anatomy and chemical composition of calcium storage organelles in their native state and at nanometer-scale resolution. We show that the dense calcium phase inside the calcium storage compartment of the calcifying coccolithophore Pleurochrysis carterae and the calcium phase stored in acidocalcisomes of the noncalcifying alga Chlamydomonas reinhardtii have common features. Our observations suggest that this strategy for concentrating calcium is a widespread trait and has been adapted for coccolith formation. The link we describe between acidocalcisomal calcium storage and calcium storage in coccolithophores implies that our physiological and molecular genetic understanding of acidocalcisomes could have relevance to the calcium pathway underlying coccolithophore calcification, offering a fresh entry point for mechanistic investigations on the adaptability of this process to changing oceanic conditions. |
英文关键词 | acidocalcisome biomineralization intracellular calcium store coccolithophore cryo-X-ray tomography |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000448040500064 |
WOS关键词 | X-RAY MICROSCOPY ; CRICOSPHAERA CARTERAE ; POLYPHOSPHATE BODIES ; ACIDOCALCISOMES ; EVOLUTION ; PHOSPHATE ; BEAMLINE ; ORIGIN ; CELL |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/205002 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany; 2.Max Planck Inst Colloids & Interfaces, Dept Biomat, D-14476 Potsdam, Germany; 3.ALBA Synchrotron Light Source, Barcelona 08290, Spain; 4.Weizmann Inst Sci, Dept Struct Biol, IL-76100 Rehovot, Israel; 5.Aix Marseille Univ, Commissariat Energie Atom & Energies Alternat CEA, CNRS,UMR 7265, Inst Biosci & Biotechnol,Lab Bioenerget Cellulair, F-13108 St Paul Les Durance, France; 6.Weizmann Inst Sci, Dept Plant & Environm Sci, IL-76100 Rehovot, Israel |
推荐引用方式 GB/T 7714 | Gal, Assaf,Sorrentino, Andrea,Kahil, Keren,et al. Native-state imaging of calcifying and noncalcifying microalgae reveals similarities in their calcium storage organelles[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(43):11000-11005. |
APA | Gal, Assaf,Sorrentino, Andrea,Kahil, Keren,Pereiro, Eva,Faivre, Damien,&Scheffel, Andre.(2018).Native-state imaging of calcifying and noncalcifying microalgae reveals similarities in their calcium storage organelles.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(43),11000-11005. |
MLA | Gal, Assaf,et al."Native-state imaging of calcifying and noncalcifying microalgae reveals similarities in their calcium storage organelles".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.43(2018):11000-11005. |
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