Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1073/pnas.1608462114 |
Two distinct pools of B-12 analogs reveal community interdependencies in the ocean | |
Heal, Katherine R.1; Qin, Wei2; Ribalet, Francois1; Bertagnolli, Anthony D.2,5; Coyote-Maestas, Willow1,6; Hmelo, Laura R.1; Moffett, James W.3,4; Devol, Allan H.1; Armbrust, E. Virginia1; Stahl, David A.2; Ingalls, Anitra E.1 | |
2017-01-10 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
出版年 | 2017 |
卷号 | 114期号:2页码:364-369 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Organisms within all domains of life require the cofactor cobalamin (vitamin B-12), which is produced only by a subset of bacteria and archaea. On the basis of genomic analyses, cobalamin biosynthesis in marine systems has been inferred in three main groups: select heterotrophic Proteobacteria, chemoautotrophic Thaumarchaeota, and photoautotrophic Cyanobacteria. Culture work demonstrates that many Cyanobacteria do not synthesize cobalamin but rather produce pseudocobalamin, challenging the connection between the occurrence of cobalamin biosynthesis genes and production of the compound in marine ecosystems. Here we show that cobalamin and pseudocobalamin coexist in the surface ocean, have distinct microbial sources, and support different enzymatic demands. Even in the presence of cobalamin, Cyanobacteria synthesize pseudocobalaminlikely reflecting their retention of an oxygen-independent pathway to produce pseudocobalamin, which is used as a cofactor in their specialized methionine synthase (MetH). This contrasts a model diatom, Thalassiosira pseudonana, which transported pseudocobalamin into the cell but was unable to use pseudocobalamin in its homolog of MetH. Our genomic and culture analyses showed that marine Thaumarchaeota and select heterotrophic bacteria produce cobalamin. This indicates that cobalamin in the surface ocean is a result of de novo synthesis by heterotrophic bacteria or via modification of closely related compounds like cyanobacterially produced pseudocobalamin. Deeper in the water column, our study implicates Thaumarchaeota as major producers of cobalamin based on genomic potential, cobalamin cell quotas, and abundance. Together, these findings establish the distinctive roles played by abundant prokaryotes in cobalamin-based microbial interdependencies that sustain community structure and function in the ocean. |
英文关键词 | B-12 cobalamin Thaumarchaeota pseudocobalamin Cyanobacteria |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000391439300053 |
WOS关键词 | AMMONIA-OXIDIZING ARCHAEA ; DEPENDENT METHIONINE SYNTHASE ; MARINE-BACTERIA ; LOWER LIGAND ; RIBONUCLEOTIDE REDUCTASE ; SALMONELLA-ENTERICA ; PSEUDOVITAMIN B-12 ; ESCHERICHIA-COLI ; PHYTOPLANKTON ; VITAMIN-B-12 |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204714 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Washington, Sch Oceanog, Seattle, WA 98195 USA; 2.Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA; 3.Univ Southern Calif, Dept Biol Sci, Los Angeles, CA 90089 USA; 4.Univ Southern Calif, Dept Civil & Environm Engn, Los Angeles, CA 90089 USA; 5.Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA; 6.Univ Minnesota, Dept Biochem Biophys & Mol Biol, Minneapolis, MN 55455 USA |
推荐引用方式 GB/T 7714 | Heal, Katherine R.,Qin, Wei,Ribalet, Francois,et al. Two distinct pools of B-12 analogs reveal community interdependencies in the ocean[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(2):364-369. |
APA | Heal, Katherine R..,Qin, Wei.,Ribalet, Francois.,Bertagnolli, Anthony D..,Coyote-Maestas, Willow.,...&Ingalls, Anitra E..(2017).Two distinct pools of B-12 analogs reveal community interdependencies in the ocean.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(2),364-369. |
MLA | Heal, Katherine R.,et al."Two distinct pools of B-12 analogs reveal community interdependencies in the ocean".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.2(2017):364-369. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论