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DOI | 10.1038/s41467-019-12783-5 |
The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining | |
Li, Conglei1; Irrazabal, Thergiory1; So, Clare C.1; Berru, Maribel1; Du, Likun2; Lam, Evelyn1; Ling, Alexanda K.1; Gommerman, Jennifer L.1; Pan-Hammarstrom, Qiang2; Martin, Alberto1 | |
2019-10-30 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Canada; Sweden |
英文摘要 | Class switch recombination (CSR) has a fundamental function during humoral immune response and involves the induction and subsequent repair of DNA breaks in the immunoglobulin (Ig) switch regions. Here we show the role of Usp22, the SAGA complex deubiquitinase that removes ubiquitin from H2B-K120, in the repair of programmed DNA breaks in vivo. Ablation of Usp22 in primary B cells results in defects in gamma H2AX and impairs the classical non-homologous end joining (c-NHEJ), affecting both V(D)J recombination and CSR. Surprisingly, Usp22 depletion causes defects in CSR to various Ig isotypes, but not IgA. We further demonstrate that IgG CSR primarily relies on c-NHEJ, whereas CSR to IgA is more reliant on the alternative end joining pathway, indicating that CSR to different isotypes involves distinct DNA repair pathways. Hence, Usp22 is the first deubiquitinase reported to regulate both V(D)J recombination and CSR in vivo by facilitating c-NHEJ. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000427007700015 |
WOS关键词 | HISTONE H2B ; V(D)J RECOMBINATION ; LIGASE-IV ; B-CELLS ; PRO-B ; AID ; REPAIR ; 53BP1 ; PATHWAYS ; CHAIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204624 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Toronto, Dept Immunol, Toronto, ON M5S 1A8, Canada; 2.Karolinska Inst, Dept Lab Med, SE-14186 Stockholm, Sweden |
推荐引用方式 GB/T 7714 | Li, Conglei,Irrazabal, Thergiory,So, Clare C.,et al. The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Li, Conglei.,Irrazabal, Thergiory.,So, Clare C..,Berru, Maribel.,Du, Likun.,...&Martin, Alberto.(2019).The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining.NATURE COMMUNICATIONS,9. |
MLA | Li, Conglei,et al."The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining".NATURE COMMUNICATIONS 9(2019). |
条目包含的文件 | 条目无相关文件。 |
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