Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-12499-6 |
Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro | |
Lombardo, Andrew T.1; Nelson, Shane R.1; Ali, M. Yusuf1; Kennedy, Guy G.1; Trybus, Kathleen M.1; Walcott, Sam2; Warshaw, David M.1 | |
2019-10-15 | |
发表期刊 | NATURE COMMUNICATIONS
![]() |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Intracellular cargo transport relies on myosin Va molecular motor ensembles to travel along the cell's three-dimensional (3D) highway of actin filaments. At actin filament intersections, the intersecting filament is a structural barrier to and an alternate track for directed cargo transport. Here we use 3D super-resolution fluorescence imaging to determine the directional outcome (that is, continues straight, turns or terminates) for an similar to 10 motor ensemble transporting a 350 nm lipid-bound cargo that encounters a suspended 3D actin filament intersection in vitro. Motor-cargo complexes that interact with the intersecting filament go straight through the intersection 62% of the time, nearly twice that for turning. To explain this, we develop an in silico model, supported by optical trapping data, suggesting that the motors' diffusive movements on the vesicle surface and the extent of their engagement with the two intersecting actin tracks biases the motor-cargo complex on average to go straight through the intersection. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000402533900001 |
WOS关键词 | HAND-OVER-HAND ; STEPPING KINETICS ; PROCESSIVITY ; TAIL ; MICROSCOPY ; TRACKING ; BILAYERS ; MOTION ; MODEL ; LOAD |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204595 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Vermont, Dept Mol Physiol & Biophys, Burlington, VT 05405 USA; 2.Univ Calif Davis, Dept Math, Davis, CA 95616 USA |
推荐引用方式 GB/T 7714 | Lombardo, Andrew T.,Nelson, Shane R.,Ali, M. Yusuf,et al. Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Lombardo, Andrew T..,Nelson, Shane R..,Ali, M. Yusuf.,Kennedy, Guy G..,Trybus, Kathleen M..,...&Warshaw, David M..(2019).Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro.NATURE COMMUNICATIONS,8. |
MLA | Lombardo, Andrew T.,et al."Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论