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DOI | 10.1038/s41467-019-12473-2 |
FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons | |
Errichelli, Lorenzo1,2; Modigliani, Stefano Dini1; Laneve, Pietro1; Colantoni, Alessio2; Legnini, Ivano2; Capauto, Davide1,2; Rosa, Alessandro1,2; De Santis, Riccardo1,2; Scarfo, Rebecca2; Peruzzi, Giovanna1; Lu, Lei3; Caffarelli, Elisa1,4; Shneider, Neil A.3; Morlando, Mariangela2; Bozzoni, Irene1,2,4,5 | |
2019-09-30 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Italy; USA |
英文摘要 | The RNA-binding protein FUS participates in several RNA biosynthetic processes and has been linked to the pathogenesis of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. Here we report that FUS controls back-splicing reactions leading to circular RNA (circRNA) production. We identified circRNAs expressed in in vitro-derived mouse motor neurons (MNs) and determined that the production of a considerable number of these circRNAs is regulated by FUS. Using RNAi and overexpression of wild-type and ALS-associated FUS mutants, we directly correlate the modulation of circRNA biogenesis with alteration of FUS nuclear levels and with putative toxic gain of function activities. We also demonstrate that FUS regulates circRNA biogenesis by binding the introns flanking the back-splicing junctions and that this control can be reproduced with artificial constructs. Most circRNAs are conserved in humans and specific ones are deregulated in human-induced pluripotent stem cell-derived MNs carrying the FUSP525L mutation associated with ALS. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000397796000001 |
WOS关键词 | AMYOTROPHIC-LATERAL-SCLEROSIS ; EXON CIRCULARIZATION ; SR PROTEINS ; ALS ; MUTATIONS ; GENE ; DISEASES ; FUS/TLS ; SMN ; DIFFERENTIATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204569 |
专题 | 资源环境科学 |
作者单位 | 1.Ist Italiano Tecnol, Ctr Life Nano Sci Sapienza, Viale Regina Elena 291, I-00161 Rome, Italy; 2.Sapienza Univ Rome, Deparment Biol & Biotechnol Charles Darwin, Ple A Moro 5, I-00185 Rome, Italy; 3.Columbia Univ, Dept Neurol, Ctr Motor Neuron Biol & Dis, 630 W 168th St, New York, NY 10032 USA; 4.Sapienza Univ Rome, CNR, Inst Mol Biol & Pathol, Ple A Moro 5, I-00185 Rome, Italy; 5.Sapienza Univ Rome, Inst Pasteur, Fdn Cenci Bolognetti, Ple A Moro 5, I-00185 Rome, Italy |
推荐引用方式 GB/T 7714 | Errichelli, Lorenzo,Modigliani, Stefano Dini,Laneve, Pietro,et al. FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Errichelli, Lorenzo.,Modigliani, Stefano Dini.,Laneve, Pietro.,Colantoni, Alessio.,Legnini, Ivano.,...&Bozzoni, Irene.(2019).FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons.NATURE COMMUNICATIONS,8. |
MLA | Errichelli, Lorenzo,et al."FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
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