Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-12062-3 |
Megahertz serial crystallography | |
Wiedorn, Max O.1,2,3; Oberthuer, Dominik1; Bean, Richard4; Schubert, Robin3,5,6; Werner, Nadine5; Abbey, Brian7; Aepfelbacher, Martin8; Adriano, Luigi9; Allahgholi, Aschkan9; Al-Qudami, Nasser4; Andreasson, Jakob10,11,12; Aplin, Steve1; Awel, Salah1,3; Ayyer, Kartik1; Bajt, Sasa9; Barak, Imrich1,3; Bari, Sadia9; Bielecki, Johan4; Botha, Sabine3,5; Boukhelef, Djelloul4; Brehm, Wolfgang1; Brockhauser, Sandor4,14; Cheviakov, Igor8; Coleman, Matthew A.15; Cruz-Mazo, Francisco16; Danilevski, Cyril4; Darmanin, Connie7; Doak, R. Bruce17; Domaracky, Martin1; Doerner, Katerina4; Du, Yang1; Fangohr, Hans4,18; Fleckenstein, Holger1; Frank, Matthias15; Fromme, Petra19; Ganan-Calvo, Alfonso M.16; Gevorkov, Yaroslav1,20; Giewekemeyer, Klaus4; Ginn, Helen Mary21,22,23; Graafsma, Heinz9,24; Graceffa, Rita4; Greiffenberg, Dominic25; Gumprecht, Lars1; Goettlicher, Peter9; Hajdu, Janos10,11; Hauf, Steffen4; Heymann, Michael26; Holmes, Susannah7; Horke, Daniel A.1,3; Hunter, Mark S.27; Imlau, Siegfried1; Kaukher, Alexander4; Kim, Yoonhee4; Klyuev, Alexander9; Knoska, Juraj1,2; Kobe, Bostjan28,29; Kuhn, Manuela9; Kupitz, Christopher30; Kuepper, Jochen1,2,3,31; Lahey-Rudolph, Janine Mia1,32; Laurus, Torsten9; Le Cong, Karoline5; Letrun, Romain4; Xavier, P. Lourdu1,33; Maia, Luis4; Maia, Filipe R. N. C.10,34; Mariani, Valerio1; Messerschmidt, Marc4; Metz, Markus1; Mezza, Davide25; Michelat, Thomas4; Mills, Grant4; Monteiro, Diana C. F.3; Morgan, Andrew1; Muhlig, Kerstin10; Munke, Anna10; Muennich, Astrid4; Nette, Julia3; Nugent, Keith A.7; Nuguid, Theresa5; Orville, Allen M.22,23; Pandey, Suraj30; Pena, Gisel1; Villanueva-Perez, Pablo1; Poehlsen, Jennifer9; Previtali, Gianpietro4; Redecke, Lars8,32; Riekehr, Winnie Maria32; Rohde, Holger8; Round, Adam4; Safenreiter, Tatiana1; Sarrou, Iosifina1; Sato, Tokushi1,4; Schmidt, Marius30; Schmitt, Bernd25; Schoenherr, Robert32; Schulz, Joachim4; Sellberg, Jonas A.35; Seibert, M. Marvin10; Seuring, Carolin13; Shelby, Megan L.15; Shoeman, Robert L.17; Sikorski, Marcin4; Silenzi, Alessandro4; Stan, Claudiu A.36; Shi, Xintian25; Stern, Stephan1,4; Sztuk-Dambietz, Jola4; Szuba, Janusz4; Tolstikova, Aleksandra1; Trebbin, Martin3,37,38; Trunk, Ulrich9; Vagovic, Patrik1,4; Ve, Thomas39; Weinhausen, Britta4; White, Thomas A.1; Wrona, Krzysztof4; Xu, Chen4; Yefanov, Oleksandr1; Zatsepin, Nadia40; Zhang, Jiaguo25; Perbandt, Markus3,5,8; Mancuso, Adrian P.4; Betzel, Christian3,5,6; Chapman, Henry1,2,3; Barty, Anton1 | |
2019-09-20 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Australia; Sweden; Czech Republic; Slovakia; Hungary; USA; Spain; England; Switzerland |
英文摘要 | The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a beta-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000446016400003 |
WOS关键词 | M BETA-LACTAMASES ; STRUCTURE REFINEMENT ; RAY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204550 |
专题 | 资源环境科学 |
作者单位 | 1.DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany; 2.Univ Hamburg, Dept Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany; 3.Univ Hamburg, Hamburg Ctr Ultrafast Imaging, Luruper Chaussee 149, D-22761 Hamburg, Germany; 4.European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany; 5.Univ Hamburg, Inst Biochem & Mol Biol, Lab Struct Biol Infect & Inflammat, Notkestr 85, D-22607 Hamburg, Germany; 6.Integrated Biol Infrastruct Life Sci Facil Europe, Holzkoppel 4, D-22869 Schenefeld, Germany; 7.La Trobe Univ, Ctr Excellence Adv Mol Imaging, La Trobe Inst Mol Sci, Dept Chem & Phys,ARC, Bundoora, Vic 3086, Australia; 8.Univ Med Ctr Hamburg Eppendorf UKE, Inst Med Microbiol Virol & Hyg, D-20246 Hamburg, Germany; 9.DESY, Notkestr 85, D-22607 Hamburg, Germany; 10.Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, S-75124 Uppsala, Sweden; 11.Czech Acad Sci, ELI Beamlines, Inst Phys, Na Slovance 2, Prague 18221, Czech Republic; 12.Chalmers Univ Technol, Dept Phys, Condensed Matter Phys, S-41296 Gothenburg, Sweden; 13.SAS, Inst Mol Biol, Dubravska Cesta 21, Bratislava 84551, Slovakia; 14.Hungarian Acad Sci, BRC, Temesvari Krt 62, H-6726 Szeged, Hungary; 15.Lawrence Livermore Natl Lab, 7000 East Ave, Livermore, CA 94550 USA; 16.Univ Seville, Dept Ingn Aeroesp & Mecan Fluidos ETSI, Seville 41092, Spain; 17.Max Planck Inst Med Res, Jahnstr 29, D-69120 Heidelberg, Germany; 18.Univ Southampton, Engn & Environm, Southampton SO17 1BJ, Hants, England; 19.Arizona State Univ, Sch Mol Sci & Biodesign, Ctr Appl Struct Discovery, Tempe, AZ 85287 USA; 20.Hamburg Univ Technol, Vis Syst E2, Harburger Schlostr 20, D-21079 Hamburg, Germany; 21.Div Struct Biol, Oxford OX3 7BN, England; 22.Diamond Light Source, Res Complex Harwell, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England; 23.Univ Oxford, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England; 24.Mid Sweden Univ, Holmgatan 10, S-85170 Sundsvall, Sweden; 25.Paul Scherrer Inst, Forsch Str 111, CH-5232 Villigen, Switzerland; 26.Max Planck Inst Biochem, Dept Cellular & Mol Biophys, D-82152 Martinsried, Germany; 27.SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA; 28.Univ Queensland, Inst Mol Biosci, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia; 29.Univ Queensland, Australian Infect Dis Res Ctr, Brisbane, Qld 4072, Australia; 30.Univ Wisconsin, Phys Dept, 3135 N Maryland Ave, Milwaukee, WI 53211 USA; 31.Univ Hamburg, Dept Chem, Martin Luther King Pl 6, D-20146 Hamburg, Germany; 32.Univ Lubeck, Inst Biochem, Ctr Struct & Cell Biol Med, Ratzeburger Allee 160, D-23562 Lubeck, Germany; 33.Max Planck Inst Struct & Dynam Matter, Luruper Chaussee 149, D-22761 Hamburg, Germany; 34.Lawrence Berkeley Natl Lab, NERSC, Berkeley, CA 94720 USA; 35.KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden; 36.Rutgers Univ Newark, Phys Dept, Newark, NJ 07102 USA; 37.Univ Buffalo, Dept Chem, 359 Nat Sci Complex, Buffalo, NY 14260 USA; 38.Univ Hamburg, Inst Nanostruct & Solid State Phys, Dept Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany; 39.Griffith Univ, Inst Glyc, Southport, Qld 4222, Australia; 40.Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA |
推荐引用方式 GB/T 7714 | Wiedorn, Max O.,Oberthuer, Dominik,Bean, Richard,et al. Megahertz serial crystallography[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Wiedorn, Max O..,Oberthuer, Dominik.,Bean, Richard.,Schubert, Robin.,Werner, Nadine.,...&Barty, Anton.(2019).Megahertz serial crystallography.NATURE COMMUNICATIONS,9. |
MLA | Wiedorn, Max O.,et al."Megahertz serial crystallography".NATURE COMMUNICATIONS 9(2019). |
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