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DOI | 10.1038/s41467-019-11822-5 |
Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis | |
Xu, Wei1,3; Luthra, Priya2; Wu, Chao1; Batra, Jyoti2; Leung, Daisy W.1; Basler, Christopher F.2; Amarasinghe, Gaya K.1 | |
2019-09-03 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | Ebola virus (EBOV) is an enveloped negative-sense RNA virus that causes sporadic outbreaks with high case fatality rates. Ebola viral protein 30 (eVP30) plays a critical role in EBOV transcription initiation at the nucleoprotein (eNP) gene, with additional roles in the replication cycle such as viral assembly. However, the mechanistic basis for how eVP30 functions during the virus replication cycle is currently unclear. Here we define a key interaction between eVP30 and a peptide derived from eNP that is important to facilitate interactions leading to the recognition of the RNA template. We present crystal structures of the eVP30 C-terminus in complex with this eNP peptide. Functional analyses of the eVP30-eNP interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30-eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000402867600001 |
WOS关键词 | C-TERMINAL DOMAIN ; MARBURG-VIRUS ; TRANSCRIPTION ; PHOSPHORYLATION ; PROTEIN ; REPLICATION ; MODEL ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204520 |
专题 | 资源环境科学 |
作者单位 | 1.Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63105 USA; 2.Georgia State Univ, Inst Biomed Sci, Ctr Microbial Pathogenesis, Atlanta, GA 30303 USA; 3.Guangzhou Med Univ, Guangzhou Peoples Hosp 8, State Key Lab Resp Dis, Guangzhou 510060, Guangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Wei,Luthra, Priya,Wu, Chao,et al. Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Xu, Wei.,Luthra, Priya.,Wu, Chao.,Batra, Jyoti.,Leung, Daisy W..,...&Amarasinghe, Gaya K..(2019).Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis.NATURE COMMUNICATIONS,8. |
MLA | Xu, Wei,et al."Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis".NATURE COMMUNICATIONS 8(2019). |
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