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DOI | 10.1038/s41467-019-10719-7 |
Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells | |
Shrestha, Roshan L.1,4; Conti, Duccio1,2; Tamura, Naoka1,5; Braun, Dominique1; Ramalingam, Revathy A.2; Cieslinski, Konstanty3; Ries, Jonas3; Draviam, Viji M.1,2 | |
2019-06-28 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | England; Germany; USA |
英文摘要 | Human chromosomes are captured along microtubule walls (lateral attachment) and then tethered to microtubule-ends (end-on attachment) through a multi-step end-on conversion process. Upstream regulators that orchestrate this remarkable change in the plane of kinetochore-microtubule attachment in human cells are not known. By tracking kinetochore movements and using kinetochore markers specific to attachment status, we reveal a spatially defined role for Aurora-B kinase in retarding the end-on conversion process. To understand how Aurora-B activity is counteracted, we compare the roles of two outer-kinetochore bound phosphatases and find that BubR1-associated PP2A, unlike KNL1-associated PP1, plays a significant role in end-on conversion. Finally, we uncover a novel role for Aurora-B regulated Astrin-SKAP complex in ensuring the correct plane of kinetochore-microtubule attachment. Thus, we identify Aurora-B as a key upstream regulator of end-on conversion in human cells and establish a late role for Astrin-SKAP complex in the end-on conversion process. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000406597700001 |
WOS关键词 | SPINDLE ASSEMBLY CHECKPOINT ; CHROMOSOME SEGREGATION ; OUTER KINETOCHORE ; MITOTIC-SPINDLE ; B56-PP2A PHOSPHATASE ; INNER CENTROMERE ; MITOSIS ; PROTEIN ; ORIENTATION ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204435 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England; 2.Queen Mary Univ London, Sch Biol & Chem Sci, London E1 4NS, England; 3.European Mol Biol Lab, Cell Biol & Biophys Unit, Meyerhofstr 1, Heidelberg, Germany; 4.NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA; 5.Queen Mary Univ London, Barts Canc Inst, London EC1M 6BQ, England |
推荐引用方式 GB/T 7714 | Shrestha, Roshan L.,Conti, Duccio,Tamura, Naoka,et al. Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Shrestha, Roshan L..,Conti, Duccio.,Tamura, Naoka.,Braun, Dominique.,Ramalingam, Revathy A..,...&Draviam, Viji M..(2019).Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.NATURE COMMUNICATIONS,8. |
MLA | Shrestha, Roshan L.,et al."Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
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