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DOI | 10.1038/s41467-019-10520-6 |
TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC | |
Kim, Dong Hyun1,2; Han, Joo Seok3; Ly, Peter1,2; Ye, Qiaozhen2; McMahon, Moira A.1,2,6; Myung, Kyungjae3,4; Corbett, Kevin D.2,5; Cleveland, Don W.1,2 | |
2019-06-21 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; South Korea |
英文摘要 | The mitotic checkpoint ensures accurate chromosome segregation through assembly of the mitotic checkpoint complex (MCC), a soluble inhibitor of the anaphase-promoting complex/cyclosome (APC/C) produced by unattached kinetochores. MCC is also assembled during interphase by Mad1/Mad2 bound at nuclear pores, thereby preventing premature mitotic exit prior to kinetochore maturation and checkpoint activation. Using degron tagging to rapidly deplete the AAA+ ATPase TRIP13, we show that its catalytic activity is required to maintain a pool of open-state Mad2 for MCC assembly, thereby supporting mitotic checkpoint activation, but is also required for timely mitotic exit through catalytic disassembly of MCC. Strikingly, combining TRIP13 depletion with elimination of APC15-dependent Cdc20 ubiquitination/degradation results in a complete inability to exit mitosis, even when MCC assembly at unattached kinetochores is prevented. Thus, mitotic exit requires MCC produced either in interphase or mitosis to be disassembled by TRIP13-catalyzed removal of Mad2 or APC15-driven ubiquitination/degradation of its Cdc20 subunit. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000447697100012 |
WOS关键词 | SPINDLE-ASSEMBLY CHECKPOINT ; ANAPHASE-PROMOTING COMPLEX ; AAA-ATPASE ; CRYSTAL-STRUCTURE ; BUDDING YEAST ; PROTEIN MAD2 ; CDC20 ; APC/C ; INACTIVATION ; INHIBITOR |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204422 |
专题 | 资源环境科学 |
作者单位 | 1.Ludwig Inst Canc Res, San Diego Branch, La Jolla, CA 92093 USA; 2.Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA; 3.Inst Basic Sci, Ctr Genom Integr, Ulsan 44919, South Korea; 4.UNIST, Sch Life Sci, Ulsan 44919, South Korea; 5.Univ Calif San Diego, Dept Chem, La Jolla, CA 92093 USA; 6.Ionis Pharmaceut, 2855 Gazelle Ct, Carlsbad, CA 92010 USA |
推荐引用方式 GB/T 7714 | Kim, Dong Hyun,Han, Joo Seok,Ly, Peter,et al. TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Kim, Dong Hyun.,Han, Joo Seok.,Ly, Peter.,Ye, Qiaozhen.,McMahon, Moira A..,...&Cleveland, Don W..(2019).TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC.NATURE COMMUNICATIONS,9. |
MLA | Kim, Dong Hyun,et al."TRIP13 and APC15 drive mitotic exit by turnover of interphase- and unattached kinetochore-produced MCC".NATURE COMMUNICATIONS 9(2019). |
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