Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-10633-y |
A rapid and robust method for single cell chromatin accessibility profiling | |
Chen, Xi1; Miragaia, Ricardo J.1,4; Natarajan, Kedar Nath1,5; Teichmann, Sarah A.1,2,3 | |
2019-06-13 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | England; Denmark |
英文摘要 | The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, very few studies have been performed at the single cell level (scATAC-seq) due to technical challenges. Here we developed a simple and robust plate-based scATAC-seq method, combining upfront bulk Tn5 tagging with single-nuclei sorting. We demonstrate that our method works robustly across various systems, including fresh and cryopreserved cells from primary tissues. By profiling over 3000 splenocytes, we identify distinct immune cell types and reveal cell type-specific regulatory regions and related transcription factors. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000453441300013 |
WOS关键词 | T-BET ; TRANSPOSITION ; MEMORY ; TRANSCRIPTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204408 |
专题 | 资源环境科学 |
作者单位 | 1.Wellcome Sanger Inst, Wellcome Genome Campus, Cambridge CB10 1SA, England; 2.EMBL European Bioinformat Inst, Wellcome Trust Genome Campus, Cambridge CB10 1SD, England; 3.Cavendish Lab, Theory Condensed Matter, 19 JJ Thomson Ave, Cambridge CB3 0HE, England; 4.MedImmune, Sir Aaron Klug Bldg,Granta Pk, Cambridge CB21 6GH, England; 5.Univ So Denmark, Funct Biol & Metab Unit, Biochem & Mol Biol, DK-5230 Odense, Denmark |
推荐引用方式 GB/T 7714 | Chen, Xi,Miragaia, Ricardo J.,Natarajan, Kedar Nath,et al. A rapid and robust method for single cell chromatin accessibility profiling[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Chen, Xi,Miragaia, Ricardo J.,Natarajan, Kedar Nath,&Teichmann, Sarah A..(2019).A rapid and robust method for single cell chromatin accessibility profiling.NATURE COMMUNICATIONS,9. |
MLA | Chen, Xi,et al."A rapid and robust method for single cell chromatin accessibility profiling".NATURE COMMUNICATIONS 9(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论