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DOI | 10.1038/s41467-019-10605-2 |
Short-chain ketone production by engineered polyketide synthases in Streptomyces albus | |
Yuzawa, Satoshi1,2,11; Mirsiaghi, Mona1,3; Jocic, Renee1; Fujii, Tatsuya2,4; Masson, Fabrice1,3; Benites, Veronica T.1,2; Baidoo, Edward E. K.1,2; Sundstrom, Eric1,3; Tanjore, Deepti1,3; Pray, Todd R.1,3; George, Anthe2,5; Davis, Ryan W.5; Gladden, John M.2,5; Simmons, Blake A.1,2; Katz, Leonard2,6; Keasling, Jay D.1,2,6,7,8,9,10,11 | |
2019-06-07 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Japan; Denmark; Peoples R China |
英文摘要 | Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Here we report engineered microbes that produce various short-chain (C5-C7) ketones using hybrid PKSs. Introduction of the genes into the chromosome of Streptomyces albus enables it to produce >1 g . l(-1) of C6 and C7 ethyl ketones and several hundred mg . l(-1) of C5 and C6 methyl ketones from plant biomass hydrolysates. Engine tests indicate these short-chain ketones can be added to gasoline as oxygenates to increase the octane of gasoline. Together, it demonstrates the efficient and renewable microbial production of biogasolines by hybrid enzymes. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000448940900001 |
WOS关键词 | BIOSYNTHETIC GENE-CLUSTER ; HIGH-LEVEL PRODUCTION ; ESCHERICHIA-COLI ; MICROBIAL-PRODUCTION ; ALPHA-LIPOMYCIN ; IN-VITRO ; E. COLI ; PATHWAY ; CHEMICALS ; ALCOHOLS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204400 |
专题 | 资源环境科学 |
作者单位 | 1.Lawrence Berkeley Natl Lab, Biog Syst & Engn Div, Berkeley, CA 94720 USA; 2.Joint BioEnegy Inst, Emeryville, CA 94608 USA; 3.Lawrence Berkeley Natl Lab, Adv Biofuels & Bioprod Proc Dev Unit, Berkeley, CA 94720 USA; 4.Natl Inst Adv Ind Sci & Technol, Inst Synthet Biol, Res Inst Sustainable Chem, Higashihiroshima, Hiroshima 7390046, Japan; 5.Sandia Natl Labs, Dept Biomass Sci & Convers Technol, Livermore, CA 94551 USA; 6.Univ Calif Berkeley, Inst QB3, Berkeley, CA 94720 USA; 7.Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA; 8.Univ Calif Berkeley, Dept Chem & Biomol Engn, Berkeley, CA 94720 USA; 9.Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Bldg 220, DK-2800 Lyngby, Denmark; 10.Shenzhen Inst Adv Technol, Ctr Synthet Biochem, Shenzhen 518055, Guangdong, Peoples R China; 11.Univ Tokyo, Biotechnol Res Ctr, Tokyo 1138657, Japan |
推荐引用方式 GB/T 7714 | Yuzawa, Satoshi,Mirsiaghi, Mona,Jocic, Renee,et al. Short-chain ketone production by engineered polyketide synthases in Streptomyces albus[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Yuzawa, Satoshi.,Mirsiaghi, Mona.,Jocic, Renee.,Fujii, Tatsuya.,Masson, Fabrice.,...&Keasling, Jay D..(2019).Short-chain ketone production by engineered polyketide synthases in Streptomyces albus.NATURE COMMUNICATIONS,9. |
MLA | Yuzawa, Satoshi,et al."Short-chain ketone production by engineered polyketide synthases in Streptomyces albus".NATURE COMMUNICATIONS 9(2019). |
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