Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-10250-9 |
Electrostatic melting in a single-molecule field-effect transistor with applications in genomic identification | |
Vernick, Sefi1; Trocchia, Scott M.1; Warren, Steven B.1; Young, Erik F.1; Bouilly, Delphine2; Gonzalez, Ruben L.2; Nuckolls, Colin2; Shepard, Kenneth L.1 | |
2019-06-03 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The study of biomolecular interactions at the single-molecule level holds great potential for both basic science and biotechnology applications. Single-molecule studies often rely on fluorescence-based reporting, with signal levels limited by photon emission from single optical reporters. The point-functionalized carbon nanotube transistor, known as the single-molecule field-effect transistor, is a bioelectronics alternative based on intrinsic molecular charge that offers significantly higher signal levels for detection. Such devices are effective for characterizing DNA hybridization kinetics and thermodynamics and enabling emerging applications in genomic identification. In this work, we show that hybridization kinetics can be directly controlled by electrostatic bias applied between the device and the surrounding electrolyte. We perform the first single-molecule experiments demonstrating the use of electrostatics to control molecular binding. Using bias as a proxy for temperature, we demonstrate the feasibility of detecting various concentrations of 20-nt target sequences from the Ebolavirus nucleoprotein gene in a constant-temperature environment. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000401510300001 |
WOS关键词 | DNA HYBRIDIZATION KINETICS ; LIQUID/SOLID INTERFACES ; SURFACE-DIFFUSION ; CARBON NANOTUBES ; NUCLEIC-ACIDS ; OLIGONUCLEOTIDES ; TRANSITIONS ; MICROARRAYS ; ADSORPTION ; DEPENDENCE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204391 |
专题 | 资源环境科学 |
作者单位 | 1.Columbia Univ, Dept Elect Engn, New York, NY 10027 USA; 2.Columbia Univ, Dept Chem, New York, NY 10027 USA |
推荐引用方式 GB/T 7714 | Vernick, Sefi,Trocchia, Scott M.,Warren, Steven B.,et al. Electrostatic melting in a single-molecule field-effect transistor with applications in genomic identification[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Vernick, Sefi.,Trocchia, Scott M..,Warren, Steven B..,Young, Erik F..,Bouilly, Delphine.,...&Shepard, Kenneth L..(2019).Electrostatic melting in a single-molecule field-effect transistor with applications in genomic identification.NATURE COMMUNICATIONS,8. |
MLA | Vernick, Sefi,et al."Electrostatic melting in a single-molecule field-effect transistor with applications in genomic identification".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论