Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells

doi:10.1038/s41467-019-09424-2

GSTDTAP > 资源环境科学

DOI	10.1038/s41467-019-09424-2
	Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells
	Cencioni, Chiara 1,2; Spallotta, Francesco 1; Savoia, Matteo 1,3; Kuenne, Carsten 4; Guenther, Stefan 4; Re, Agnese 2; Wingert, Susanne 5,6; Rehage, Maike 5,6; Sueruen, Duran 5,6; Siragusa, Mauro 7; Smith, Jacob G.8; Schnuetgen, Frank 5,6; von Melchner, Harald 5,6; Rieger, Michael A.5,6; Martelli, Fabio 9; Riccio, Antonella 8; Fleming, Ingrid 7; Braun, Thomas 10; Zeiher, Andreas M.11; Farsetti, Antonella 2,11; Gaetano, Carlo 1,12
	2019-04-02
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
出版年	2018
卷号	9
文章类型	Article
语种	英语
国家	Germany; Italy; England
英文摘要	Nitric oxide (NO) synthesis is a late event during differentiation of mouse embryonic stem cells (mESC) and occurs after release from serum and leukemia inhibitory factor (LIF). Here we show that after release from pluripotency, a subpopulation of mESC, kept in the naive state by 2i/LIF, expresses endothelial nitric oxide synthase (eNOS) and endogenously synthesizes NO. This eNOS/NO-positive subpopulation (ESNO+) expresses mesendodermal markers and is more efficient in the generation of cardiovascular precursors than eNOS/NO-negative cells. Mechanistically, production of endogenous NO triggers rapid Hdac2 S-nitrosylation, which reduces association of Hdac2 with the transcriptional repression factor Zeb1, allowing mesendodermal gene expression. In conclusion, our results suggest that the interaction between Zeb1, Hdac2, and eNOS is required for early mesendodermal differentiation of naive mESC.
领域	资源环境
收录类别	SCI-E
WOS记录号	WOS:000428621300005
WOS关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; NITRIC-OXIDE SYNTHASE ; SELECTIVE-INHIBITION ; TRANSCRIPTION FACTOR ; S-NITROSYLATION ; GROUND-STATE ; ZINC-FINGER ; DIFFERENTIATION ; EXPRESSION ; CHROMATIN
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/204298
专题	资源环境科学
作者单位	1.Goethe Univ, Dept Cardiol, Div Cardiovasc Epigenet, Theodor Stern Kai 7, D-60590 Frankfurt, Germany; 2.CNR, Inst Cell Biol & Neurobiol IBCN, Via Fosso Fiorano 64, I-00143 Rome, Italy; 3.Univ Cattolica Roma, Inst Med Pathol, Largo Francesco Vito 1, I-00168 Rome, Italy; 4.Max Planck Inst Heart & Lung Res, ECCPS Bioinformat & Deep Sequencing Platform, Ludwigstr 43, D-61231 Bad Nauheim, Germany; 5.Goethe Univ, LOEWE Ctr Cell & Gene Therapy, Theodor Stern Kai 7, D-60590 Frankfurt, Germany; 6.Goethe Univ, Dept Med Hematol Oncol, Theodor Stern Kai 7, D-60590 Frankfurt, Germany; 7.Goethe Univ, Ctr Mol Med, Inst Vasc Signalling, Theodor Stern Kai 7, D-60590 Frankfurt, Germany; 8.UCL, Lab Mol Cell Biol, MRC, Gower St, London WC1E 6BT, England; 9.Policlin San Donato, IRCCS, Mol Cardiol Lab, Via Morandi 30 San Donato Milanese, I-20097 Milan, Italy; 10.Max Planck Inst Heart & Lung Res, Dept Cardiac Dev & Remodeling, Ludwigstr 43, D-61231 Bad Nauheim, Germany; 11.Goethe Univ, Dept Cardiol, Internal Med Clin 3, Theodor Stern Kai 7, D-60590 Frankfurt, Germany; 12.Ist Clin Sci Maugeri, Lab Epigenet, Via Maugeri 4, I-27100 Pavia, Italy
推荐引用方式 GB/T 7714	Cencioni, Chiara,Spallotta, Francesco,Savoia, Matteo,et al. Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells[J]. NATURE COMMUNICATIONS,2019,9.
APA	Cencioni, Chiara.,Spallotta, Francesco.,Savoia, Matteo.,Kuenne, Carsten.,Guenther, Stefan.,...&Gaetano, Carlo.(2019).Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells.NATURE COMMUNICATIONS,9.
MLA	Cencioni, Chiara,et al."Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells".NATURE COMMUNICATIONS 9(2019).