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DOI | 10.1038/s41467-019-09448-8 |
Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes | |
Ferguson, Gabriel B.1; Van Handel, Ben1; Bay, Maxwell2; Fiziev, Petko3,4,5; Org, Tonis6,7; Lee, Siyoung1; Shkhyan, Ruzanna1; Banks, Nicholas W.1; Scheinberg, Mila1; Wu, Ling8; Saitta, Biagio1; Elphingstone, Joseph1; Larson, A. Noelle9,10; Riester, Scott M.9,10; Pyle, April D.5; Bernthal, Nicholas M.11; Mikkola, Hanna K. A.5,6; Ernst, Jason4,5,12,13,14; van Wijnen, Andre J.9,10; Bonaguidi, Michael2; Evseenko, Denis1,2,11 | |
2019-03-29 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Estonia |
英文摘要 | Tissue-specific gene expression defines cellular identity and function, but knowledge of early human development is limited, hampering application of cell-based therapies. Here we profiled 5 distinct cell types at a single fetal stage, as well as chondrocytes at 4 stages in vivo and 2 stages during in vitro differentiation. Network analysis delineated five tissue-specific gene modules; these modules and chromatin state analysis defined broad similarities in gene expression during cartilage specification and maturation in vitro and in vivo, including early expression and progressive silencing of muscle- and bone-specific genes. Finally, ontogenetic analysis of freshly isolated and pluripotent stem cell-derived articular chondrocytes identified that integrin alpha 4 defines 2 subsets of functionally and molecularly distinct chondrocytes characterized by their gene expression, osteochondral potential in vitro and proliferative signature in vivo. These analyses provide new insight into human musculoskeletal development and provide an essential comparative resource for disease modeling and regenerative medicine. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000444014100009 |
WOS关键词 | LARGE GENE LISTS ; CARTILAGE REGENERATION ; INTEGRATIVE ANALYSIS ; ADHESION MOLECULE ; PROGENITOR-CELL ; MYOGENIC CELLS ; BONE-FORMATION ; ANIMAL-MODELS ; GROWTH-PLATE ; INTEGRIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204291 |
专题 | 资源环境科学 |
作者单位 | 1.USC, Keck Sch Med, Dept Orthopaed Surg, Los Angeles, CA 90033 USA; 2.USC, Dept Stem Cell Res & Regenerat Med, Los Angeles, CA 90033 USA; 3.Univ Calif Los Angeles, Bioinformat Interdepartmental Program, Los Angeles, CA 90095 USA; 4.Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90095 USA; 5.Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA 90095 USA; 6.Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA; 7.Univ Tartu, Inst Mol & Cell Biol, EE-51010 Tartu, Estonia; 8.InVitro Cell Res LLC, Cockeysville, MD 21030 USA; 9.Mayo Clin, Dept Orthoped Surg, Ctr Regenerat Med, Rochester, MN 55905 USA; 10.Mayo Clin, Dept Biochem & Mol Biol, Ctr Regenerat Med, Rochester, MN 55905 USA; 11.Univ Calif Los Angeles, David Geffen Sch Med, Dept Orthopaed Surg, Los Angeles, CA 90095 USA; 12.Univ Calif Los Angeles, Comp Sci Dept, Los Angeles, CA 90095 USA; 13.Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA; 14.Univ Calif Los Angeles, Mol Biol Inst, Los Angeles, CA 90095 USA |
推荐引用方式 GB/T 7714 | Ferguson, Gabriel B.,Van Handel, Ben,Bay, Maxwell,et al. Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Ferguson, Gabriel B..,Van Handel, Ben.,Bay, Maxwell.,Fiziev, Petko.,Org, Tonis.,...&Evseenko, Denis.(2019).Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes.NATURE COMMUNICATIONS,9. |
MLA | Ferguson, Gabriel B.,et al."Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes".NATURE COMMUNICATIONS 9(2019). |
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