Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-09254-2 |
The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development | |
Schumacher, Maria A.1; den Hengst, Chris D.2; Bush, Matthew J.2; Le, T. B. K.2; Tran, Ngat T.2; Chandra, Govind2; Zeng, Wenjie1; Travis, Brady1; Brennan, Richard G.1; Buttner, Mark J.2 | |
2019-03-27 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England |
英文摘要 | Streptomycetes are notable for their complex life cycle and production of most clinically important antibiotics. A key factor that controls entry into development and the onset of antibiotic production is the 68-residue protein, BldC. BldC is a putative DNA-binding protein related to MerR regulators, but lacks coiled-coil dimerization and effector-binding domains characteristic of classical MerR proteins. Hence, the molecular function of the protein has been unclear. Here we show that BldC is indeed a DNA-binding protein and controls a regulon that includes other key developmental regulators. Intriguingly, BldC DNA-binding sites vary significantly in length. Our BldC-DNA structures explain this DNA-binding capability by revealing that BldC utilizes a DNA-binding mode distinct from MerR and other known regulators, involving asymmetric head-to-tail oligomerization on DNA direct repeats that results in dramatic DNA distortion. Notably, BldC-like proteins radiate throughout eubacteria, establishing BldC as the founding member of a new structural family of regulators. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000427698700008 |
WOS关键词 | TRANSCRIPTION FACTOR ; CRYSTAL-STRUCTURE ; CELL-DIVISION ; SPORULATION ; COELICOLOR ; FAMILY ; SEQUENCES ; INSIGHTS ; DOMAIN ; MEMBER |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204289 |
专题 | 资源环境科学 |
作者单位 | 1.Duke Univ, Sch Med, Dept Biochem, Durham, NC 27710 USA; 2.John Innes Ctr, Dept Mol Microbiol, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England |
推荐引用方式 GB/T 7714 | Schumacher, Maria A.,den Hengst, Chris D.,Bush, Matthew J.,et al. The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Schumacher, Maria A..,den Hengst, Chris D..,Bush, Matthew J..,Le, T. B. K..,Tran, Ngat T..,...&Buttner, Mark J..(2019).The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.NATURE COMMUNICATIONS,9. |
MLA | Schumacher, Maria A.,et al."The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development".NATURE COMMUNICATIONS 9(2019). |
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