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DOI | 10.1038/s41467-019-09265-z |
Quantifying absolute addressability in DNA origami with molecular resolution | |
Strauss, Maximilian T.1,2,3; Schueder, Florian1,2,3; Haas, Daniel1,2,3; Nickels, Philipp C.1,2,3; Jungmann, Ralf1,2,3 | |
2019-03-20 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Self-assembled DNA nanostructures feature an unprecedented addressability with sub-nanometer precision and accuracy. This addressability relies on the ability to attach functional entities to single DNA strands in these structures. The efficiency of this attachment depends on two factors: incorporation of the strand of interest and accessibility of this strand for downstream modification. Here we use DNA-PAINT super-resolution microscopy to quantify both incorporation and accessibility of all individual strands in DNA origami with molecular resolution. We find that strand incorporation strongly correlates with the position in the structure, ranging from a minimum of 48% on the edges to a maximum of 95% in the center. Our method offers a direct feedback for the rational refinement of the design and assembly process of DNA nanostructures and provides a long sought-after quantitative explanation for efficiencies of DNA-based nanomachines. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430541900016 |
WOS关键词 | SUPERRESOLUTION MICROSCOPY ; NANOSCALE SHAPES ; FOLDING DNA ; NANOSTRUCTURES ; RECONSTRUCTION ; FLUORESCENCE ; SIMULATION ; DESIGN ; MOTOR |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204268 |
专题 | 资源环境科学 |
作者单位 | 1.Ludwig Maximilians Univ Munchen, Dept Phys, D-80539 Munich, Germany; 2.Ludwig Maximilians Univ Munchen, Ctr Nanosci, D-80539 Munich, Germany; 3.Max Planck Inst Biochem, D-82152 Martinsried, Germany |
推荐引用方式 GB/T 7714 | Strauss, Maximilian T.,Schueder, Florian,Haas, Daniel,et al. Quantifying absolute addressability in DNA origami with molecular resolution[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Strauss, Maximilian T.,Schueder, Florian,Haas, Daniel,Nickels, Philipp C.,&Jungmann, Ralf.(2019).Quantifying absolute addressability in DNA origami with molecular resolution.NATURE COMMUNICATIONS,9. |
MLA | Strauss, Maximilian T.,et al."Quantifying absolute addressability in DNA origami with molecular resolution".NATURE COMMUNICATIONS 9(2019). |
条目包含的文件 | 条目无相关文件。 |
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