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DOI | 10.1038/s41467-019-08435-3 |
Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression | |
Kar, Gozde1; Kim, Jong Kyoung1,2; Kolodziejczyk, Aleksandra A.1,3; Natarajan, Kedar Nath1,3; Triglia, Elena Torlai4; Mifsud, Borbala5,6,7; Elderkin, Sarah8; Marioni, John C.1,3,9; Pombo, Ana4; Teichmann, Sarah A.1,3 | |
2019-02-14 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | England; South Korea; Germany |
英文摘要 | Polycomb repressive complexes (PRCs) are important histone modifiers, which silence gene expression; yet, there exists a subset of PRC-bound genes actively transcribed by RNA polymerase II (RNAPII). It is likely that the role of Polycomb repressive complex is to dampen expression of these PRC-active genes. However, it is unclear how this flipping between chromatin states alters the kinetics of transcription. Here, we integrate histone modifications and RNAPII states derived from bulk ChIP-seq data with single-cell RNA-sequencing data. We find that Polycomb repressive complex-active genes have greater cell-to-cell variation in expression than active genes, and these results are validated by knockout experiments. We also show that PRC-active genes are clustered on chromosomes in both two and three dimensions, and interactions with active enhancers promote a stabilization of gene expression noise. These findings provide new insights into how chromatin regulation modulates stochastic gene expression and transcriptional bursting, with implications for regulation of pluripotency and development. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000404026900006 |
WOS关键词 | EMBRYONIC STEM-CELLS ; RNA-POLYMERASE-II ; MOUSE ES CELLS ; SINGLE-CELL ; REGULATORY CIRCUITRY ; SEQUENCING DATA ; GENOME-WIDE ; BURST SIZE ; PLURIPOTENT ; CHROMATIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204223 |
专题 | 资源环境科学 |
作者单位 | 1.EBI, EMBL, Wellcome Trust Genome Campus, Cambridge CB10 1SD, England; 2.DGIST, Dept New Biol, Daegu 42988, South Korea; 3.Wellcome Trust Sanger Inst, Wellcome Trust Genome Campus, Cambridge CB10 1SA, England; 4.Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Epigenet Regulat & Chromatin Architecture Grp, Robert Roessle Str, D-13125 Berlin, Germany; 5.Canc Res UK London Res Inst, 44 Lincolns Inn Fields, London WC2A 3LY, England; 6.UCL, Dept Genet Evolut & Environm, Gower St, London WC1E 6BT, England; 7.Queen Mary Univ London, William Harvey Res Inst, Charterhouse Sq, London EC1M 6BQ, England; 8.Babraham Inst, Nucl Dynam Programme, Babraham Res Campus, Cambridge CB22 3AT, England; 9.Univ Cambridge, Li Ka Shing Ctr, Canc Res UK Cambridge Inst, Robinson Way, Cambridge CB2 0RE, England |
推荐引用方式 GB/T 7714 | Kar, Gozde,Kim, Jong Kyoung,Kolodziejczyk, Aleksandra A.,et al. Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Kar, Gozde.,Kim, Jong Kyoung.,Kolodziejczyk, Aleksandra A..,Natarajan, Kedar Nath.,Triglia, Elena Torlai.,...&Teichmann, Sarah A..(2019).Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression.NATURE COMMUNICATIONS,8. |
MLA | Kar, Gozde,et al."Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
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