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DOI | 10.1038/s41467-019-08577-4 |
Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells | |
Vertesy, Abel1,2,3; Arindrarto, Wibowo4; Roost, Matthias S.5; Reinius, Bjorn6; Torrens-Juaneda, Vanessa5; Bialecka, Monika5; Moustakas, Ioannis4,5; Ariyurek, Yavuz7; Kuijk, Ewart3; Mei, Hailiang4; Sandberg, Rickard6; van Oudenaarden, Alexander1,2,3; Lopes, Susana M. Chuva de Sousa5,8 | |
2019-02-11 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands; Sweden; Belgium |
英文摘要 | In contrast to mouse, human female germ cells develop asynchronously. Germ cells transition to meiosis, erase genomic imprints, and reactivate the X chromosome. It is unknown if these events all appear asynchronously, and how they relate to each other. Here we combine exome sequencing of human fetal and maternal tissues with single-cell RNA-sequencing of five donors. We reconstruct full parental haplotypes and quantify changes in parental allele-specific expression, genome-wide. First we distinguish primordial germ cells (PGC), pre-meiotic, and meiotic transcriptional stages. Next we demonstrate that germ cells from various stages monoallelically express imprinted genes and confirm this by methylation patterns. Finally, we show that roughly 30% of the PGCs are still reactivating their inactive X chromosome and that this is related to transcriptional stage rather than fetal age. Altogether, we uncover the complexity and cell-to-cell heterogeneity of transcriptional and epigenetic remodeling in female human germ cells. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000431960400011 |
WOS关键词 | X-CHROMOSOME INACTIVATION ; EMBRYONIC STEM-CELLS ; LONG NONCODING RNAS ; DNA-SEQUENCING DATA ; GENOME ; GENES ; FATE ; FRAMEWORK ; DYNAMICS ; DOMAIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204211 |
专题 | 资源环境科学 |
作者单位 | 1.Royal Netherlands Acad Arts & Sci, Hubrecht Inst KNAW, NL-3584 CT Utrecht, Netherlands; 2.Univ Med Ctr, NL-3584 CT Utrecht, Netherlands; 3.Univ Med Ctr Utrecht, Canc Genom Netherlands, Ctr Mol Med, Dept Genet, NL-3584 CG Utrecht, Netherlands; 4.Leiden Univ, Med Ctr, Sequencing Anal Support Core, Dept Biomed Data Sci, NL-2333 ZC Leiden, Netherlands; 5.Leiden Univ, Med Ctr, Dept Anat & Embryol, NL-2333 ZC Leiden, Netherlands; 6.Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden; 7.Leiden Univ, Med Ctr, Leiden Genome Technol Ctr, NL-2333 ZC Leiden, Netherlands; 8.Ghent Univ Hosp, Dept Reprod Med, B-9000 Ghent, Belgium |
推荐引用方式 GB/T 7714 | Vertesy, Abel,Arindrarto, Wibowo,Roost, Matthias S.,et al. Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Vertesy, Abel.,Arindrarto, Wibowo.,Roost, Matthias S..,Reinius, Bjorn.,Torrens-Juaneda, Vanessa.,...&Lopes, Susana M. Chuva de Sousa.(2019).Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells.NATURE COMMUNICATIONS,9. |
MLA | Vertesy, Abel,et al."Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells".NATURE COMMUNICATIONS 9(2019). |
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