GSTDTAP  > 资源环境科学
DOI10.1038/s41467-018-08017-9
Rapid and reversible epigenome editing by endogenous chromatin regulators
Braun, Simon M. G.1,2; Kirkland, Jacob G.1,2; Chory, Emma J.1,2,3; Husmann, Dylan1,2; Calarco, Joseph P.1,2; Crabtree, Gerald R.1,2,4
2019-01-10
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2017
卷号8
文章类型Article
语种英语
国家USA
英文摘要

Understanding the causal link between epigenetic marks and gene regulation remains a central question in chromatin biology. To edit the epigenome we developed the FIRE-Cas9 system for rapid and reversible recruitment of endogenous chromatin regulators to specific genomic loci. We enhanced the dCas9-MS2 anchor for genome targeting with Fkbp/Frb dimerizing fusion proteins to allow chemical-induced proximity of a desired chromatin regulator. We find that mSWI/SNF (BAF) complex recruitment is sufficient to oppose Polycomb within minutes, leading to activation of bivalent gene transcription in mouse embryonic stem cells. Furthermore, Hp1/Suv39h1 heterochromatin complex recruitment to active promoters deposits H3K9me3 domains, resulting in gene silencing that can be reversed upon washout of the chemical dimerizer. This inducible recruitment strategy provides precise kinetic information to model epigenetic memory and plasticity. It is broadly applicable to mechanistic studies of chromatin in mammalian cells and is particularly suited to the analysis of endogenous multi-subunit chromatin regulator complexes.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000410812200007
WOS关键词TRANSCRIPTIONAL ACTIVATION ; GENE-EXPRESSION ; HISTONE H3 ; STEM-CELLS ; LYSINE 9 ; GENOME ; COMPLEX ; HETEROCHROMATIN ; PLURIPOTENT ; MODULATION
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
URL查看原文
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/204154
专题资源环境科学
作者单位1.Stanford Univ, Dept Pathol, Sch Med, Stanford, CA 94305 USA;
2.Stanford Univ, Dept Dev Biol, Sch Med, Stanford, CA 94305 USA;
3.Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA;
4.Howard Hughes Med Inst, Chevy Chase, MD 20815 USA
推荐引用方式
GB/T 7714
Braun, Simon M. G.,Kirkland, Jacob G.,Chory, Emma J.,et al. Rapid and reversible epigenome editing by endogenous chromatin regulators[J]. NATURE COMMUNICATIONS,2019,8.
APA Braun, Simon M. G.,Kirkland, Jacob G.,Chory, Emma J.,Husmann, Dylan,Calarco, Joseph P.,&Crabtree, Gerald R..(2019).Rapid and reversible epigenome editing by endogenous chromatin regulators.NATURE COMMUNICATIONS,8.
MLA Braun, Simon M. G.,et al."Rapid and reversible epigenome editing by endogenous chromatin regulators".NATURE COMMUNICATIONS 8(2019).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Braun, Simon M. G.]的文章
[Kirkland, Jacob G.]的文章
[Chory, Emma J.]的文章
百度学术
百度学术中相似的文章
[Braun, Simon M. G.]的文章
[Kirkland, Jacob G.]的文章
[Chory, Emma J.]的文章
必应学术
必应学术中相似的文章
[Braun, Simon M. G.]的文章
[Kirkland, Jacob G.]的文章
[Chory, Emma J.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。