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DOI | 10.1038/s41467-018-07915-2 |
Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection | |
Xin, Gang1; Zander, Ryan1; Schauder, David M.2; Chen, Yao2; Weinstein, Jason S.3; Drobyski, William R.2; Tarakanova, Vera2; Craft, Joseph3; Cui, Weiguo1,2 | |
2019-01-10 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | During chronic viral infection, the inflammatory function of CD4 T-cells becomes gradually attenuated. Concurrently, Th1 cells progressively acquire the capacity to secrete the cytokine IL-10, a potent suppressor of antiviral T cell responses. To determine the transcriptional changes that underlie this adaption process, we applied a single-cell RNA-sequencing approach and assessed the heterogeneity of IL-10-expressing CD4 T-cells during chronic infection. Here we show an IL-10-producing population with a robust Tfh-signature. Using IL-10 and IL-21 double-reporter mice, we further demonstrate that IL-10(+)IL-21(+)co-producing Tfh cells arise predominantly during chronic but not acute LCMV infection. Importantly, depletion of IL-10(+)IL-21(+)co-producing CD4 T-cells or deletion of Il10 specifically in Tfh cells results in impaired humoral immunity and viral control. Mechanistically, B cell-intrinsic IL-10 signaling is required for sustaining germinal center reactions. Thus, our findings elucidate a critical role for Tfh-derived IL-10 in promoting humoral immunity during persistent viral infection. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000451433800007 |
WOS关键词 | T-FOLLICULAR-HELPER ; CHRONIC VIRAL-INFECTION ; FACTOR C-MAF ; GERMINAL-CENTER ; CUTTING EDGE ; IL-10 PRODUCTION ; DIFFERENTIATION ; IL-27 ; CD4 ; EXPRESSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204153 |
专题 | 资源环境科学 |
作者单位 | 1.BloodCtr Wisconsin, Blood Res Inst, Milwaukee, WI 53213 USA; 2.Med Coll Wisconsin, Dept Microbiol & Mol Genet, Milwaukee, WI 53226 USA; 3.Yale Univ, Sch Med, Dept Immunobiol, 333 Cedar St, New Haven, CT 06520 USA |
推荐引用方式 GB/T 7714 | Xin, Gang,Zander, Ryan,Schauder, David M.,et al. Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Xin, Gang.,Zander, Ryan.,Schauder, David M..,Chen, Yao.,Weinstein, Jason S..,...&Cui, Weiguo.(2019).Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection.NATURE COMMUNICATIONS,9. |
MLA | Xin, Gang,et al."Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection".NATURE COMMUNICATIONS 9(2019). |
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