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DOI | 10.1038/s41467-018-07992-3 |
Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission | |
Schoeneberg, Johannes1,5; Lehmann, Martin2,3; Ullrich, Alexander1; Posor, York2,3; Lo, Wen-Ting2; Lichtner, Gregor1,2; Schmoranzer, Jan2,3,6; Haucke, Volker2,3,4; Noe, Frank1 | |
2019-01-08 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; USA |
英文摘要 | Clathrin-mediated endocytosis (CME) involves membrane-associated scaffolds of the bin-amphiphysin-rvs (BAR) domain protein family as well as the GTPase dynamin, and is accompanied and perhaps triggered by changes in local lipid composition. How protein recruitment, scaffold assembly and membrane deformation is spatiotemporally controlled and coupled to fission is poorly understood. We show by computational modelling and super-resolution imaging that phosphatidylinositol 3,4-bisphosphate [PI(3,4)P-2] synthesis within the clathrin-coated area of endocytic intermediates triggers selective recruitment of the PX-BAR domain protein SNX9, as a result of complex interactions of endocytic proteins competing for phospholipids. The specific architecture induces positioning of SNX9 at the invagination neck where its self-assembly regulates membrane constriction, thereby providing a template for dynamin fission. These data explain how lipid conversion at endocytic pits couples local membrane constriction to fission. Our work demonstrates how computational modelling and super-resolution imaging can be combined to unravel function and mechanisms of complex cellular processes. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000403603300001 |
WOS关键词 | PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE ; CLATHRIN ADAPTER ; PLASMA-MEMBRANE ; DYNAMIN ; DIFFUSION ; PROTEINS ; ACTIN ; SIMULATION ; PHOSPHOINOSITIDES ; CURVATURE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204150 |
专题 | 资源环境科学 |
作者单位 | 1.Free Univ Berlin, Dept Math & Comp Sci, D-14195 Berlin, Germany; 2.Leibniz Inst Mol Pharmacol, Robert Roessle Str 10, D-13125 Berlin, Germany; 3.Free Univ Berlin, Fac Biol Chem Pharm, D-14195 Berlin, Germany; 4.Charite Univ Med Berlin, NeuroCure Cluster Excellence, Virchowweg 6, D-10117 Berlin, Germany; 5.Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA; 6.Charite Univ Med Berlin, Virchowweg 6, D-10117 Berlin, Germany |
推荐引用方式 GB/T 7714 | Schoeneberg, Johannes,Lehmann, Martin,Ullrich, Alexander,et al. Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Schoeneberg, Johannes.,Lehmann, Martin.,Ullrich, Alexander.,Posor, York.,Lo, Wen-Ting.,...&Noe, Frank.(2019).Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission.NATURE COMMUNICATIONS,8. |
MLA | Schoeneberg, Johannes,et al."Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission".NATURE COMMUNICATIONS 8(2019). |
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