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DOI | 10.1038/s41467-018-07549-4 |
SMRT-Cappable-seq reveals complex operon variants in bacteria | |
Yan, Bo1; Boitano, Matthew2; Clark, Tyson A.2; Ettwiller, Laurence1 | |
2018-09-10 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Current methods for genome-wide analysis of gene expression require fragmentation of original transcripts into small fragments for short-read sequencing. In bacteria, the resulting fragmented information hides operon complexity. Additionally, in vivo processing of transcripts confounds the accurate identification of the 5' and 3' ends of operons. Here we develop a methodology called SMRT-Cappable-seq that combines the isolation of unfragmented primary transcripts with single-molecule long read sequencing. Applied to E. coli, this technology results in an accurate definition of the transcriptome with 34% of known operons from RegulonDB being extended by at least one gene. Furthermore, 40% of transcription termination sites have read-through that alters the gene content of the operons. As a result, most of the bacterial genes are present in multiple operon variants reminiscent of eukaryotic splicing. By providing such granularity in the operon structure, this study represents an important resource for the study of prokaryotic gene network and regulation. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000444078300019 |
WOS关键词 | ESCHERICHIA-COLI ; TRANSCRIPTION TERMINATION ; RNA-POLYMERASE ; GENE-REGULATION ; MECHANISMS ; DEGRADATION ; SEQUENCES ; INSIGHTS ; STRAINS ; BIOLOGY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204108 |
专题 | 资源环境科学 |
作者单位 | 1.New England Biolabs Inc, 240 Cty Rd, Ipswich, MA 01938 USA; 2.PacBio, 1305 OBrien Dr, Menlo Pk, CA 94025 USA |
推荐引用方式 GB/T 7714 | Yan, Bo,Boitano, Matthew,Clark, Tyson A.,et al. SMRT-Cappable-seq reveals complex operon variants in bacteria[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Yan, Bo,Boitano, Matthew,Clark, Tyson A.,&Ettwiller, Laurence.(2018).SMRT-Cappable-seq reveals complex operon variants in bacteria.NATURE COMMUNICATIONS,9. |
MLA | Yan, Bo,et al."SMRT-Cappable-seq reveals complex operon variants in bacteria".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
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