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DOI | 10.1038/s41467-018-07426-0 |
KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation | |
Campbell, Mel1; Watanabe, Tadashi2; Nakano, Kazushi1; Davis, Ryan R.3; Lyu, Yuanzhi1; Tepper, Clifford G.4; Durbin-Johnsons, Blythe5; Fujimuro, Masahiro2; Izumiya, Yoshihiro1,4,6 | |
2018-01-04 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Japan |
英文摘要 | The three-dimensional structure of chromatin organized by genomic loops facilitates RNA polymerase II access to distal promoters. The Kaposi's sarcoma-associated herpesvirus (KSHV) lytic transcriptional program is initiated by a single viral transactivator, K-Rta. Here we report the KSHV genomic structure and its relationship with K-Rta recruitment sites using Capture Hi-C analyses. High-resolution 3D viral genomic maps identify a number of direct physical, long-range, and dynamic genomic interactions. Mutant KSHV chromosomes harboring point mutations in the K-Rta responsive elements (RE) significantly attenuate not only the directly proximate downstream gene, but also distal gene expression in a domain-specific manner. Genomic loops increase in the presence of K-Rta, while abrogation of K-Rta binding impairs the formation of inducible genomic loops, decreases the expression of genes networked through the looping, and diminishes KSHV replication. Our study demonstrates that genomic architectural dynamics plays an essential role in herpesvirus gene expression. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000419404100001 |
WOS关键词 | SARCOMA-ASSOCIATED HERPESVIRUS ; KAPOSIS-SARCOMA ; HIGH-RESOLUTION ; EXPRESSION ; PROTEIN ; LATENCY ; GENOME ; TRANSCRIPTION ; VIRUS ; CHROMOSOME |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204102 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Calif Davis, Sch Med, Dept Dermatol, Sacramento, CA 95817 USA; 2.Kyoto Pharmaceut Univ, Dept Cell Biol, Kyoto 6078412, Japan; 3.Univ Calif Davis, Dept Pathol & Lab Med, Sch Med, Sacramento, CA 95817 USA; 4.Univ Calif Davis, Dept Biochem & Mol Med, Sch Med, Sacramento, CA 95817 USA; 5.Univ Calif Davis, Dept Publ Hlth Sci, Div Biostat, Sch Med, Sacramento, CA 95817 USA; 6.Univ Calif Davis, Comprehens Canc Ctr, Sacramento, CA 95817 USA |
推荐引用方式 GB/T 7714 | Campbell, Mel,Watanabe, Tadashi,Nakano, Kazushi,et al. KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Campbell, Mel.,Watanabe, Tadashi.,Nakano, Kazushi.,Davis, Ryan R..,Lyu, Yuanzhi.,...&Izumiya, Yoshihiro.(2018).KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation.NATURE COMMUNICATIONS,9. |
MLA | Campbell, Mel,et al."KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation".NATURE COMMUNICATIONS 9(2018). |
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