Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-018-07226-6 |
A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport | |
Zhang, Yuqin1; Nasser, Victoria1; Pisanty, Odelia1; Omary, Moutasem1; Wulff, Nikolai2; Di Donato, Martin3; Tal, Iris1; Hauser, Felix4; Hao, Pengchao3; Roth, Ohad1; Fromm, Hillel1; Schroeder, Julian I.4; Geisler, Markus3; Nour-Eldin, Hussam Hassan2; Shani, Eilon1 | |
2018-10-11 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Israel; Denmark; Switzerland; USA |
英文摘要 | Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000447074200002 |
WOS关键词 | PLANT DEVELOPMENT ; SECONDARY METABOLITES ; PLASMA-MEMBRANE ; EFFLUX ; GROWTH ; MAINTENANCE ; PROTEIN ; TRANSLOCATION ; BIOSYNTHESIS ; EXPRESSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204090 |
专题 | 资源环境科学 |
作者单位 | 1.Tel Aviv Univ, Sch Plant Sci & Food Secur, IL-69978 Tel Aviv, Israel; 2.Univ Copenhagen, Fac Sci, Dept Plant & Environm Sci, DynaMo Ctr,Copenhagen Plant Sci Ctr, DK-1871 Frederiksberg, Denmark; 3.Univ Fribourg, Dept Biol, CH-1700 Fribourg, Switzerland; 4.Univ Calif San Diego, Div Biol Sci, Cell & Dev Biol Sect, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Zhang, Yuqin,Nasser, Victoria,Pisanty, Odelia,et al. A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Zhang, Yuqin.,Nasser, Victoria.,Pisanty, Odelia.,Omary, Moutasem.,Wulff, Nikolai.,...&Shani, Eilon.(2018).A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport.NATURE COMMUNICATIONS,9. |
MLA | Zhang, Yuqin,et al."A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport".NATURE COMMUNICATIONS 9(2018). |
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